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Planta Med 2022; 88(12): 1026-1035
DOI: 10.1055/a-1690-3528
Biological and Pharmacological Activity
Original Papers

In Vitro and In Vivo Antiglycation Effects of Connarus ruber Extract

Mariko Takenokuchi 
1   Faculty of Pharmacological Sciences, Daiichi University of Pharmacy, Fukuoka, Japan
,
Kinuyo Matsumoto
2   Faculty of Health and Welfare, Kobe Womenʼs University, Kobe, Hyogo, Japan
,
Yuko Nitta
2   Faculty of Health and Welfare, Kobe Womenʼs University, Kobe, Hyogo, Japan
,
Rumi Takasugi
3   Kyodo Byori Inc., Kobe, Hyogo, Japan
,
Yukari Inoue
4   Department of Pharmaceutical Health Care, Faculty of Pharmaceutical Sciences, Himeji Dokkyo University, Himeji, Hyogo, Japan
,
Michi Iwai
5   Nippi Research Institute of Biomatrix, Toride, Ibaraki, Japan
,
Keiichi Kadoyama
4   Department of Pharmaceutical Health Care, Faculty of Pharmaceutical Sciences, Himeji Dokkyo University, Himeji, Hyogo, Japan
,
Kazutoshi Yoshida
6   Hyogo Prefectural Institute of Technology, Kobe, Hyogo, Japan
,
Hiromi Takano-Ohmuro
7   Faculty of Pharmacy, Musashino University, Nishitokyo, Tokyo, Japan
,
Taizo Taniguchi
8   Research Institute for Human Health Science, Konan University, Kobe, Hyogo, Japan.
9   Pharmacrea Kobe Co. Ltd., Kobe, Hyogo, Japan
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Abstract

Accumulation of advanced glycation end products (AGEs) of the Maillard reaction has been implicated in the pathogenesis of diabetes and its complications. Connarus ruber has been used as a folk remedy for several diseases, including diabetes; however, its underlying mechanism has not yet been investigated. This study investigated the effects of C. ruber extract against glycation on collagen-linked AGEs in vitro and streptozotocin-induced diabetic rats (STZ-DM rats) in vivo. The antiglycation activities of C. ruber extract and aminoguanidine (AG) were examined using a collagen glycation assay kit. Nonfluorescent AGE, Nε-carboxymethyl lysine (CML), Nω-carboxymethyl arginine, and Nε-carboxyethyl lysine levels were measured via electrospray ionization-liquid chromatography-tandem mass spectrometry. The effect of the extract on the cytotoxicity of methylglyoxal (MG), a precursor of AGEs, was examined in HL60 cells. STZ-DM rats were treated with the extract for 4 wk, and the effect was assessed using biochemical markers in the serum and CML-positive cells in renal tissues. C. ruber extract dose-dependently inhibited the glycation of collagen and formation of nonfluorescent AGEs, which was comparable to AG, and it significantly attenuated MG-induced cytotoxicity in HL60 cells. Furthermore, the glycated albumin levels in STZ-DM rats decreased, the increase in serum lipid levels was reversed, and immunohistochemistry demonstrated that CML deposition in the glomerulus of STZ-DM rats significantly decreased. Although further studies are needed, C. ruber could be a potential therapeutic for preventing and progressing many pathological conditions, including diabetes.

Key words

Connarus ruber - Connaraceae - antiglycation - advanced glycation end product - methylglyoxal - diabetes


Publication History

Received: 09 June 2021

Accepted after revision: 08 November 2021

Article published online:
03 December 2021

© 2021. Thieme. All rights reserved.

Georg Thieme Verlag KG
Rüdigerstraße 14, 70469 Stuttgart, Germany

 

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