Download PDF
Homeopathy 2013; 102(01): 41-48
DOI: 10.1016/j.homp.2012.11.002
Original Paper
Copyright © The Faculty of Homeopathy 2012

Dynamized follicle-stimulating hormone affects the development of ovine preantral follicles cultured in vitro

Laritza Ferreira de Lima
1   Laboratory of Manipulation of Oocytes and Preantral Follicles (LAMOFOPA), Faculty of Veterinary, State University of Ceará, Fortaleza-CE, Brazil
,
Rebeca Magalhães Pedrosa Rocha
1   Laboratory of Manipulation of Oocytes and Preantral Follicles (LAMOFOPA), Faculty of Veterinary, State University of Ceará, Fortaleza-CE, Brazil
,
Anelise Maria Costa Vasconcelos Alves
1   Laboratory of Manipulation of Oocytes and Preantral Follicles (LAMOFOPA), Faculty of Veterinary, State University of Ceará, Fortaleza-CE, Brazil
,
Márcia Viviane Alves Saraiva
1   Laboratory of Manipulation of Oocytes and Preantral Follicles (LAMOFOPA), Faculty of Veterinary, State University of Ceará, Fortaleza-CE, Brazil
,
Valdevane Rocha Araújo
1   Laboratory of Manipulation of Oocytes and Preantral Follicles (LAMOFOPA), Faculty of Veterinary, State University of Ceará, Fortaleza-CE, Brazil
,
Isadora Machado Teixeira Lima
1   Laboratory of Manipulation of Oocytes and Preantral Follicles (LAMOFOPA), Faculty of Veterinary, State University of Ceará, Fortaleza-CE, Brazil
,
Cláudio Afonso Pinho Lopes
1   Laboratory of Manipulation of Oocytes and Preantral Follicles (LAMOFOPA), Faculty of Veterinary, State University of Ceará, Fortaleza-CE, Brazil
,
Sônia Nair Báo
2   Laboratory of Electron Microscopy, Department of Cell Biology, University of Brasilia, Brasília-DF, Brazil
,
Claudio Cabral Campello
1   Laboratory of Manipulation of Oocytes and Preantral Follicles (LAMOFOPA), Faculty of Veterinary, State University of Ceará, Fortaleza-CE, Brazil
,
Ana Paula Ribeiro Rodrigues
1   Laboratory of Manipulation of Oocytes and Preantral Follicles (LAMOFOPA), Faculty of Veterinary, State University of Ceará, Fortaleza-CE, Brazil
,
José Ricardo de Figueiredo
1   Laboratory of Manipulation of Oocytes and Preantral Follicles (LAMOFOPA), Faculty of Veterinary, State University of Ceará, Fortaleza-CE, Brazil
› Author Affiliations
Subject Editor:
Further Information

Publication History

Received06 March 2012
revised25 October 2012

accepted12 November 2012

Publication Date:
29 December 2017 (online)

    Permissions and Reprints

Objective: To evaluate the effect of dynamized follicle-stimulating hormone (FSH) on the survival, activation and growth of ovine preantral follicles (PFs) in vitro.

Methods: Ovarian fragments were cultured for 1 or 7 days in alpha minimum essential medium (α-MEM+) control in the absence or presence of alcohol (Al control) or FSH (6cH, 12cH and 30cH) added at intervals of 24 or 48 h. The ovarian fragments were processed, coded and analyzed by a blinded observer by classical histology (CH), fluorescence microscopy (FM) and transmission electron microscopy (TEM).

Results: After 7 days of culture, the group which to which FSH 6cH was added at 24 h intervals showed better rates of follicle survival and activation compared to α-MEM+ control or Al control (p < 0.05). This group also showed higher follicle and oocyte growth than α-MEM+ control (p < 0.05). FM and TEM techniques confirmed that FSH 6cH promoted viability and ultrastructural integrity of follicles after 7 days of culture.

Conclusions: FSH 6cH (24 h) treatment maintained the viability, and promoted the activation and in vitro growth of ovine PFs.

Keywords

Homeopathy - Ovary - Survival - Ruminant
 

  • References

  • 1 Figueiredo J.R., Celestino J.J.H., Faustino L.R., Rodrigues A.P.R. In vitro culture of caprine preantral follicles: advances, limitations and prospects. Small Ruminant Res 2011; 98: 192-195.
    CrossrefPubMedGoogle Scholar
  • 2 Demirci B., Lornage J., Salle B., Frappart L., Franck M., Guerin J.F. Follicular viability and morphology of sheep ovaries after exposure to cryoprotectant and cryopreservation with different freezing protocols. Fertil Steril 2001; 75: 754-762.
    CrossrefPubMedGoogle Scholar
  • 3 Amorim C.A., Rondina D., Rodrigues A.P.R., Gonçalves P.B., de Figeuiredo J.R., Giorgetti A. Cryopreservation of isolated ovine primordial follicles with propylene glycol and glycerol. Fertil Steril 2004; 81: 735-740.
    CrossrefPubMedGoogle Scholar
  • 4 Nilsson E., Skinner M.K. Cellular interactions that control primordial follicle development and folliculogenesis. J Soc Gynecol Invest 2001; 8: S17-S20.
    CrossrefPubMedGoogle Scholar
  • 5 Pakarainen T., Zhang F., Nurmi L., Poutanen M., Huhtaniemi I. Knockout of luteinizing hormone receptor abolishes the effects of follicle-stimulating hormone on preovulatory maturation and ovulation of mouse graafian follicle. Mol Endocrinol 2005; 19: 2591-2602.
    CrossrefPubMedGoogle Scholar
  • 6 Thomas F.H., Ethier J.F., Shimasaki S., Vanderhyden B.C. Follicle-stimulating hormone regulates oocyte growth by modulation of expression of oocyte and granulosa cell factors. Endocrinology 2005; 146: 941-949.
    CrossrefPubMedGoogle Scholar
  • 7 Magalhães D.M., Araujo V.R., Verde I.B.L. et al. Different follicle stimulating hormone (FSH) sources influence caprine preantral follicle viability and development in vitro . Pesqui Vet Bras 2009; 46 (05) 378-386.
    PubMedGoogle Scholar
  • 8 Hahnemann S. Organon da arte de curar. São Paulo Industria Gráfica e Editora Ltda 1996 6a.Edição.
    PubMedGoogle Scholar
  • 9 Khuda-bukhsh A.R. Laboratory research in homeopathy: pro. Integr Cancer Ther 2006; 5 (04) 320-332.
    CrossrefPubMedGoogle Scholar
  • 10 Varshney J.P., Naresh R. Comparative efficacy of homeopathic and allopathic systems of medicine in the management of clinical mastitis of Indian dairy cows. Homeopathy 2005; 94 (02) 81-85.
    Article in Thieme ConnectPubMedGoogle Scholar
  • 11 Soto F.R.M., Vuaden E.R., Coelho C.D.P. et al. Randomized controlled trial of homeopathic treatment of weaned piglets in a commercial swine herd. Homeopathy 2008; 97: 202-205.
    Article in Thieme ConnectPubMedGoogle Scholar
  • 12 Alberti A.L.L., Hellmeinster Z.M.M., Santarém V., Loposy C.B. Eficácia do composto homeopático (fator ovino) no controle de nematóides gastrintestinais, em ovinos naturalmente infectados e criados a campo. São Paulo: APTA Regional Alta Sorocabana; 2004.
    Google Scholar
  • 13 Duke A., Bogenschutz-godwin J.M., Cellier J.D., Duke P.K. Hand book of medicinal herbs. 2nd edn. Boca Raton, FL: CRC Press; 2002. p. 843.
    Google Scholar
  • 14 Rajkumar R., Srivastava S.K., Yadav M.C. et al. Effect of a Homeopathic complex on oestrus induction and hormonal profile in anoestrus cows. Homeopathy 2006; 95: 131-135.
    Article in Thieme ConnectPubMedGoogle Scholar
  • 15 Castilhos L.R., Souza J.C., Pinto L.F. et al. Avaliação da Terapêutica Homeopática nas Ovariopatias Císticas de Bovinos Leiteiros. Homeo Bras 2003; 9 (01) 5-15.
    PubMedGoogle Scholar
  • 16 Legros M.S. Utilización de La homeopatía en endocrinología ginecológica. Uso terapéutico de las hormonas diluidas y dinamizadas. Rev Med Homeo 2010; 3 (01) 9-13.
    PubMedGoogle Scholar
  • 17 Matos M.H.T., Lima-verde I.B., Luque M.C.A. et al. Essential role of follicle stimulating hormone in the maintenance of caprine préantral follicle viability in vitro. Zygote 2007; 15: 173-182.
    CrossrefPubMedGoogle Scholar
  • 18 Amorim C.A., Lucci C.M., Rodrigues A.P.R. et al. Quantitative and qualitative analysis of the effectiveness of a mechanical method for the isolation of preantral follicles from ovine ovaries. Theriogenology 2000; 6: 1251-1262.
    PubMedGoogle Scholar
  • 19 Saraiva M.V.A., Celestino J.J.H., Chaves R.N. et al. Influence of different concentrations of LH and FSH on in vitro caprine primordial ovarian follicle development. Small Ruminant Res 2008; 78: 87-95.
    CrossrefPubMedGoogle Scholar
  • 20 Andrade E.R., Seneda M.M., Alfieri A.A. et al. Interactions of indole acetic acid with EGF and FSH in the culture of ovine preantral follicles. Theriogenology 2005; 64: 1104-1113.
    CrossrefPubMedGoogle Scholar
  • 21 Lopes A.C.P., Santos R.R., Celestino J.J. et al. Short-term preservation of canine preantral follicles: effects of temperature, medium and time. Anim Reprod Sci 2009; 115: 201-214.
    CrossrefPubMedGoogle Scholar
  • 22 Lucci C.M., Amorim C.A., Báo S.N. et al. Effect of the interval of serial sections of ovarian tissue in the tissue chopper on the number of isolated caprine preantral follicles. Anim Reprod Sci 1999; 56: 39-49.
    CrossrefPubMedGoogle Scholar
  • 23 Xu Z., Garverick H.A., Smith G.W., Smith M.F., Hamilton S.A., Youngguist R.S. Expression of follicle-stimulating hormone and luteinizing hormone receptor messenger ribonucleic acids in bovine follicles during the first follicular wave. Biol Reprod 1995; 53: 951-957.
    CrossrefPubMedGoogle Scholar
  • 24 Khuda-bukhsh A.R. Towards understanding molecular mechanisms of action of homeopathic drugs: an overview. Mol Cell Biochem 2003; 253: 339-345.
    CrossrefPubMedGoogle Scholar
  • 25 Lo S.Y., Li W.C., Huang S.H. Water clusters in Life. Med Hypotheses 2000; 6 (54) 948-953.
    PubMedGoogle Scholar
  • 26 Zhou K.X., Lu G.W., Zhou Q.C., Song J.H., Jiang S.T., Xia H.R. Monte Carlo simulation of liquid water in a magnetic field. J Appl Phys 2000; 88 (04) 1802-1805.
    CrossrefPubMedGoogle Scholar
  • 27 Anagnostatos G.S. Small water clusters (clathrates) in the homoeopathic preparation process. In: Endler P.C., Schulte J. (eds). Ultra High Dilution: Physiology and Physics. 1994. Dordrecht, the Netherlands: Kluwer Academic Publishers; 121-128.
    Google Scholar