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Vet Comp Orthop Traumatol 2014; 27(03): 204-209
DOI: 10.3415/VCOT-13-09-0113
Original Research
Schattauer GmbH

Survival of bone marrow mesenchymal stem cells labelled with red fluorescent protein in an ovine model of collagenase-induced tendinitis

L. Lacitignola
1   Dipartimento dell’Emergenza e dei Trapianti di Organi (D.E.T.O.), Sezione di Cliniche Veterinarie e P.A, Università degli Studi di Bari “Aldo Moro”, Valenzano, BARI, Italy
,
F. Staffieri
1   Dipartimento dell’Emergenza e dei Trapianti di Organi (D.E.T.O.), Sezione di Cliniche Veterinarie e P.A, Università degli Studi di Bari “Aldo Moro”, Valenzano, BARI, Italy
,
G. Rossi
2   Dipartimento di Scienze Veterinarie, Università degli Studi di Camerino, Facoltà di Medicina Veterinaria, Matelica (MC), Italy
,
E. Francioso
1   Dipartimento dell’Emergenza e dei Trapianti di Organi (D.E.T.O.), Sezione di Cliniche Veterinarie e P.A, Università degli Studi di Bari “Aldo Moro”, Valenzano, BARI, Italy
,
A. Crovace
1   Dipartimento dell’Emergenza e dei Trapianti di Organi (D.E.T.O.), Sezione di Cliniche Veterinarie e P.A, Università degli Studi di Bari “Aldo Moro”, Valenzano, BARI, Italy
› Author Affiliations
Further Information

Publication History

Received 15 September 2013

Accepted 09 March 2014

Publication Date:
21 December 2017 (online)

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Summary

Objective: The aim of this study was to track the survival and efficacy of allogeneic bone marrow mesenchymal stem cells (BM-MSC) marked with red fluorescent protein (BMMSCRFP) in an ovine model of collagenase-induced tendinopathy.

Methods: Bone marrow was harvested from one donor sheep and BM-MSC were isolated, cultivated and transfected with red fluorescent protein (BM-MSCRFP). Collagenase was injected into both Achilles tendons in the remaining nine sheep. After two weeks the left tendon was injected with a solution of 6 x 106 BM-MSCRFP and fibrin glue, while only fibrin glue was administered to the contralateral tendon in each sheep. After three, four and six weeks the tendons were harvested and evaluated for morphology, collagen I deposition, presence of CD34+ cells, and fluorescent labelled BM-MSC.

Results: We demonstrated that delivery of BM-MSC into tendon lesions had positive effects on the injured tendons. The BM-MSCRFP survived at three, four and six weeks after treatment, leading to better quality healing of tendons as compared to the controls, where no labelled cells were detected. Interestingly, we demonstrated high expression of CD34+ cells in tendons that had been treated with BM-MSCRFP.

Clinical relevance: Mesenchymal stem cell allografts have a positive effect on tendon healing and local injection of BM-MSC directly into the tendon allows the homing of BM-MSC for good efficiency of engraftment.

Keywords

BM-MSC - tendinopathy - Achilles tendon - red fluorescent protein - CD34+ cells - collagen I
 

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