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Thromb Haemost 2015; 114(06): 1251-1259
DOI: 10.1160/TH15-04-0357
New Technologies, Diagnostic Tools and Drugs
Schattauer GmbH

Fractal dimension (df ) as a new structural biomarker of clot microstructure in different stages of lung cancer

Nia Anne Davies
1   College of Medicine, Swansea University, Swansea, UK
2   NISCHR Haemostasis Biomedical Research Unit, Morriston Hospital, ABMU Health Board, Swansea, UK
,
Nicholas Kim Harrison
1   College of Medicine, Swansea University, Swansea, UK
3   Respiratory Unit, Morriston Hospital, ABMU Health Board, Swansea, UK
,
Roger H. Keith Morris
4   School of Applied Science, Cardiff Metropolitan University, Cardiff, UK
,
Simon Noble
5   School of Medicine, Cardiff University, Cardiff, UK
,
Matthew James Lawrence
1   College of Medicine, Swansea University, Swansea, UK
2   NISCHR Haemostasis Biomedical Research Unit, Morriston Hospital, ABMU Health Board, Swansea, UK
,
Lindsay Antonio D’Silva
1   College of Medicine, Swansea University, Swansea, UK
2   NISCHR Haemostasis Biomedical Research Unit, Morriston Hospital, ABMU Health Board, Swansea, UK
,
Laura Broome
1   College of Medicine, Swansea University, Swansea, UK
2   NISCHR Haemostasis Biomedical Research Unit, Morriston Hospital, ABMU Health Board, Swansea, UK
,
Martin Rowan Brown
6   College of Engineering, Swansea University, Swansea, UK
,
Karl M. Hawkins
1   College of Medicine, Swansea University, Swansea, UK
,
Phylip Rhodri Williams
6   College of Engineering, Swansea University, Swansea, UK
,
Simon Davidson
7   Department of Haematology, Royal Brompton Hospital, Royal Brompton and Harefield NHS Foundation Trust, London, UK
,
Phillip Adrian Evans
1   College of Medicine, Swansea University, Swansea, UK
2   NISCHR Haemostasis Biomedical Research Unit, Morriston Hospital, ABMU Health Board, Swansea, UK
› Author Affiliations Financial support: This translational work was supported by the NISCHR Biomedical Research Unit (BRU) grant (BRO1).
Further Information

Publication History

Received: 29 April 2015

Accepted after major revision: 28 June 2015

Publication Date:
30 November 2017 (online)

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Summary

Venous thromboembolism (VTE) is common in cancer patients, and is the second commonest cause of death associated with the disease. Patients with chronic inflammation, such as cancer, have been shown to have pathological clot structures with modulated mechanical properties. Fractal dimension (df) is a new technique which has been shown to act as a marker of the microstructure and mechanical properties of blood clots, and can be performed more readily than current methods such as scanning electron microscopy (SEM). We measured df in 87 consecutive patients with newly diagnosed lung cancer prior to treatment and 47 matched-controls. Mean group values were compared for all patients with lung cancer vs controls and for limited disease vs extensive disease. Results were compared with conventional markers of coagulation, fibrinolysis and SEM images. Significantly higher values of df were observed in lung cancer patients compared with controls and patients with extensive disease had higher values than those with limited disease (p< 0.05), whilst conventional markers failed to distinguish between these groups. The relationship between df of the incipient clot and mature clot microstructure was confirmed by SEM and computational modelling: higher df was associated with highly dense clots formed of smaller fibrin fibres in lung cancer patients compared to controls. This study demonstrates that df is a sensitive technique which quantifies the structure and mechanical properties of blood clots in patients with lung cancer. Our data suggests that df has the potential to identify patients with an abnormal clot micro-structure and greatest VTE risk.

Keywords

Lung cancer - venous thromboembolism - risk assessment - biomarker - fractal
 

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