Epidermal Growth Factor and Parathyroid Hormone-related Peptide mRNA in the Mammary Gland and their Concentrations in Milk: Effects of Postpartum Hypoxia in Lactating Rats

E. D. Bruder; J. Van Hoof; J. B. Young; H. Raff

doi:10.1055/s-2008-1058101

Hormone and Metabolic Research, Table of Contents

Horm Metab Res 2008; 40(7): 446-453
DOI: 10.1055/s-2008-1058101

Original Basic

Epidermal Growth Factor and Parathyroid Hormone-related Peptide mRNA in the Mammary Gland and their Concentrations in Milk: Effects of Postpartum Hypoxia in Lactating Rats

E. D. Bruder¹ , J. Van Hoof¹ ^, ² , J. B. Young³ , H. Raff¹ ^, ²

¹Endocrine Research Laboratory, Aurora St. Luke's Medical Center, Milwaukee, Wisconsin, USA
²Department of Medicine, Medical College of Wisconsin, Milwaukee, Wisconsin, USA
³Department of Medicine, Feinberg School of Medicine, Northwestern University, Chicago, Illinois, USA

Abstract

The physiological adaptations of the neonatal rat to hypoxia from birth include changes in gastrointestinal function and intermediary metabolism. We hypothesized that the hypoxic lactating dam would exhibit alterations in mammary gland function leading to changes in the concentration of milk peptides that are important in neonatal gastrointestinal development. The present study assessed the effects of chronic hypoxia on peptides produced by the mammary glands and present in milk. Chronic hypoxia decreased the concentration of epidermal growth factor (EGF) in expressed milk and pup stomach contents and decreased maternal mammary gland Egf mRNA. The concentration of parathyroid hormone-related protein (PTHrp) was unchanged in milk and decreased in pup stomach contents; however, mammary Pthlh mRNA was increased by hypoxia. There was a significant increase in adiponectin concentrations in milk from hypoxic dams. Chronic hypoxia decreased maternal body weight, and pair feeding normoxic dams an amount of food equivalent to hypoxic dam food intake decreased body weight to an equivalent degree. Decreased food intake did not affect the expression of Egf, Pthlh, or Lep mRNA in mammary tissue. The results indicated that chronic hypoxia modulated mammary function independently of hypoxia-induced decreases in maternal food intake. Decreased EGF and increased adiponectin concentrations in milk from hypoxic dams likely affect the development of neonatal intestinal function.

Key words

lactation - leptin - adiponectin - stomach contents

Full Text

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Correspondence

H. RaffPhD

Endocrinology

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