Raster- Elektronenmikroskopie an Gefäßausgüssen des Auges

Zusammenfassung

Raster-elektronenmikroskopische Untersuchung von Methylmethacrylat/Sevriton Ausgüssen erlaubt räumliche Rekonstruktion von Gefäßen des Auges. Die Arbeit zeigt die Möglichkeit von Oberflächen-Studien an kleinsten Gefäßen zur Unterscheidung zwischen Venen und Arterien in der Retina und am N. opticus des Katzenauges.

Summary

Plastic casts of the vasculature of the cat eye were obtained by injecting an acrylic mixture into the arterial afferent system by an in vivo and an in vitro procedure. The acrylic mixture traversed the capillary segment of the circulation, filling the effluent veins. The three-dimensional casts obtained could then be observed in toto or after dissection, allowing SEM inspection of the ocular vasculature from all sides. Findings concerning the distribution of veins in the optic nerve revealed an extensive plexus, particularly developed at the posterior pole and feeding two major efferents on both sides of the optic nerve. This feature is of interest to physiologists using the perfused in vitro preparation of the cat eye. Retinal vascular features observed by this method are likely to be helpful in clarifying certain anatomical substrates of autoregulatory mechanisms.