Abstract
A new secobutanolide, secoaggregatalactone A (1) was isolated from the leaves of Lindera aggregata. Results obtained from the cytotoxicity assay revealed that secoaggregatalactone
A exhibited a noticeable cytotoxicity (EC50 = 6.61 μg/mL; 22.1 μM) against the human hepatoma cell line (Hep G2 cell line). According
to morphological observations, flow cytometric analysis, and DNA fragmentation analysis,
it was proven that the cytotoxicity of secoaggregatalactone A on human cells was due
to apoptosis. Moreover, based on the results from the protein expression assay and
confocal laser scanning microscope observations, it is assumed that secoaggregatalactone
A induced apoptosis through the mitochondria pathway by way of cleavage of Bit to
release cytochrome C and activate caspases-9 and -3, and then degradation of PARP.
Key words
Secoaggregatalactone A - apoptosis - cytotoxicity -
Lindera aggregata
- Lauraceae - mitochondrial pathway
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Dr. Sheng-Yang Wang
Department of Forestry
National Chung-Hsing University
250 Kuo-Kuang Road
Taichung 402
Taiwan
R.O.C.
Phone: +886-4-2284-0345 ext. 138
Fax: +886-4-2287-3628.
Email: taiwanfir@dragon.nchu.edu.tw