Abstract
The purpose of this investigation was to examine the influence of an acute bout of
resistance exercise (RE) on intramuscular triglyceride (IMTG) and muscle glycogen
concentrations and intracellular signaling in women with high body fat content. Six
overweight women with a high percent body fat (age 29 ± 3 yr; BMI 28 ± 3 kg/m2, body fat 38 ± 4 %) performed 6 sets of 10 repetitions of knee extension exercise
at 70 % 1RM. Muscle biopsies were obtained from the vastus lateralis before, 1 min
after (POST1), and 2 h after (POST2) exercise. Acute RE reduced (p < 0.05) IMTG content
∼ 40 % at POST1 and POST2 (75 ± 5; 45 ± 6; 50 ± 10 mmol/kg/dry wt). Muscle glycogen
was also reduced (p < 0.05) ∼ 25 % at POST1 and remained lower at POST2 (317 ± 14;
241 ± 30; 235 ± 26 mmol/kg/dry wt). ERK1/2, SAPK/JNK, and p38 phosphorylation were
increased (p < 0.05) ∼ 2 – 3-fold at POST1 and ERK1/2 remained elevated and POST2
whereas SAPK/JNK and p38 returned to basal levels. AMPKα phosphorylation was unchanged
in response to RE. These results show that both IMTG and muscle glycogen stores serve
as an important energy source during RE in overweight women and the MAP kinase signaling
response to RE is not compromised by high levels of body fat.
Key words
glycogen - IMTG - ERK1/2 - MAPK - AMPK
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Dr. Matthew Harber
Ball State University
Human Performance Laboratory
2000 Unversity Ave.
47306 Muncie, Indiana
United States
Telefon: + 76 52 85 98 40
Fax: + 76 52 85 85 96
eMail: mharber@bsu.edu
