Abstract
The aim of the present study was to test the influence of obesity and the presence
of type 2 diabetes mellitus (T2DM) on the expression of ten housekeeping genes and
of the 18S rRNA in a group of human adipose tissue samples from the omental and subcutaneous
depot. Adipose tissue biopsies were obtained by laparoscopic surgery from lean and
obese patients. After the extraction, mRNA levels in adipose tissue samples were quantified
by real-time PCR using the commercial Human Endogenous Control Plates. From the genes analyzed, 18S rRNA exhibited the most stable expression levels in
both depots regardless of the pathophysiological conditions of obesity and obesity-associated
T2DM. Contrarily, GAPD was the gene with the highest variation in its expression levels,
being upregulated (8.0-fold) in the obese group and downregulated (3.5-fold) in obesity-associated
T2DM. Our results show that 18S rRNA may be the most suitable gene for normalization
in expression studies performed in human adipose tissue samples obtained from patients
suffering from obesity and/or obesity-associated T2DM, whereas GAPD is less appropriate
for comparison purposes under these circumstances.
Key words
Obesity - type 2 diabetes mellitus - real-time PCR - adipose tissue - housekeeping
gene expression
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Correspondence
G. FrühbeckR Nutr, MD, PhD
Department of Endocrinology
Clínica Universitaria de Navarra
University of Navarra
Avda. Pío XII, 36
31008 Pamplona
Spain
Phone: +34/948/25 54 00 (ext. 44 84)
Fax: +34/948/29 65 00
Email: gfruhbeck@unav.es