Protective Action of Ilex paraguariensis Extract against Free Radical Inactivation of Paraoxonase-1 in High-Density Lipoprotein

Abstract

Paraoxonase 1 (PON-1), an antioxidant enzyme carried mainly by HDL, has been shown to display cardioprotective effects. In this study, we investigated whether the polyphenol-rich beverage mate, obtained from extract of Ilex paraguariensis (IP), prevented the loss of PON-1 activity from HDL during oxidant stress. The peroxide radical generator 2,2-azobis(2-amidinopropane) dihydrochloride (AAPH) induced time- and dose-dependent oxidation of HDL, as measured by lipoperoxide content, which is accompanied by a parallel decrease in the activity of PON-1 (p < 0.001). IP extract (2 - 20 µL/mL) afforded time- and concentration-dependent inhibition of the oxidation, with preservation of apoA-I structure and PON-1 activity. Healthy volunteers drank either 0.5 L of IP extract, 0.5 L of coffee and milk or nothing. PON-1 activity increased an average of 10 % above the changes seen when the intake was coffee and milk (p < 0.05). In conclusion, we demonstrate that IP extract may, to some extent, prevent the loss of the antiatherogenic function of HDL afforded by PON-1 when the particle is under oxidant stress.

Abbreviations

ApoA-I:apolipoprotein A-I

HDL:high-density lipoprotein

LDL:low-density lipoprotein

PON-1:paraoxonase 1

Aquifoliaceae - Ilex paraguariensis - paraoxonase 1 - oxidative stress - HDL - atherosclerosis - polyphenol

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