Plaunotol Induces Apoptosis of Gastric Cancer Cells

Abstract

Although some isoprenoids, such as taxans and geranylgeraniol (GGOH), have been reported to have strong anticancer activities, the effect of plaunotol, the isoprenoid extracted from the leaves of Plau-noi, on cancer has not yet been evaluated. Here, we aimed to investigate the effect of plaunotol on gastric cancer cell lines. Three gastric cancer cell lines, namely MKN-45, MKN-74 and AZ-521 were used. Plaunotol was tested at 10, 20, 30 and 40 μmol/L. Plaunotol dose-dependently inhibited the growth of all gastric cancer cells, dependent on the induction of apoptosis. Caspases-8, -9 and -3, were found to be activated in the apoptotic cells. The expression of Bax protein was increased, but Bcl-2 and Bcl-xL protein expressions were not significantly affected. Plaunotol should be a promising new antitumor agent, and since it is already available for clinical use in Japan, its anticancer properties should be confirmed in clinical trials.

Abbreviations

COX: cyclooxygenase

DMSO: dimethyl sulfoxide

FITC: fluorescein isothiocyanate

GGOH: geranylgeraniol

MTS: 3-(4,5-dimethylthiazol-2-yl)-5-(3-carboxymethoxyphenyl)-2-(4-sulfo-phenyl)-2H-tetrazolium

PBS: phosphate-buffered saline

PG: prostaglandin

PI: propidium iodide

TUNEL: terminal deoxynucleotidyl transferase-mediated deoxyuridine triphosphate-biotin nick end labelling

Plaunotol - cell apoptosis - isoprenoids - gastric cancer

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