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Plant Biol (Stuttg) 2007; 9(4): 516-525
DOI: 10.1055/s-2007-964974
Research Paper

Georg Thieme Verlag Stuttgart KG · New York

Genetic Structure of Araucaria angustifolia (Araucariaceae) Populations in Brazil: Implications for the in situ Conservation of Genetic Resources

V. M. Stefenon1 , O. Gailing1 , R. Finkeldey1
  • 1Institute of Forest Genetics and Forest Tree Breeding, Georg August University Göttingen, Büsgenweg 2, 37077 Göttingen, Germany
Further Information

Publication History

Received: July 21, 2006

Accepted: January 12, 2007

Publication Date:
02 April 2007 (online)

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Abstract

The distribution of the genetic variation within and among natural populations of A. angustifolia growing in different regions in Brazil was assessed at microsatellite and AFLP markers. Both markers revealed high gene diversity (H = 0.65; AR = 9.1 for microsatellites and H = 0.27; P = 77.8 % for AFLPs), moderate overall differentiation (RST = 0.13 for microsatellites and FST = 0.10 for AFLPs), but high divergence of the northernmost, geographically isolated population. In a Bayesian analysis, microsatellite data suggested population structure at two levels: at K = 2 and at K = 3 in agreement to the geographical distribution of populations. This result was confirmed by the UPGMA dendrogram based on microsatellite data (bootstrap support > 95 %). Non-hierarchical AMOVA revealed high variation among populations from different a posteriori defined geographical groups. The genetic distance between sample locations increased with geographical distance for microsatellites (r = 0.62; p = 0.003) and AFLPs (r = 0.32; p = 0.09). This pattern of population differentiation may be correlated with population history such as geographical isolation and postglacial colonization of highlands. Implications of the population genetic structure for the conservation of genetic resources are discussed.

Key words

AFLPs - Araucaria angustifolia - genetic diversity - genetic resources - microsatellites - population structure - population history.

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V. M. Stefenon

Institute of Forest Genetics and Forest Tree Breeding
Georg August University Göttingen

Büsgenweg 2

37077 Göttingen

Germany

Email: gene_mol@yahoo.com.br

Editor: H. de Kroon