Effect of NSAID on Muscle Injury and Oxidative Stress

Abstract

Indirect markers of muscle damage and delayed onset muscle soreness were examined and correlated to changes in oxidative stress, plasma antioxidant potential, and use or nonuse of non-steroidal anti-inflammatory drugs in 60 ultra-marathoners following the Western States Endurance Run. Blood was collected prior to and immediately following the race and analyzed for muscle damage by creatine phosphokinase and oxidative stress by F₂-isoprostanes, protein carbonyls, and lipid hydroperoxides and antioxidant potential by the ferric reducing ability of plasma. Subjects recorded delayed onset muscle soreness during the week following the race. Lipid hydroperoxide concentrations were unchanged, but F₂-isoprostanes, protein carbonyls, ferric reducing ability of plasma, creatine phosphokinase, and delayed onset muscle soreness increased significantly postrace. Protein carbonyls were significantly higher postrace in nonsteroidal anti-inflammatory drug users versus nonusers (p < 0.05). However, there was no difference between users and non-users for all other markers. Postrace creatine phosphokinase concentrations were not correlated with oxidative stress markers but were correlated with changes in delayed onset muscle soreness. Based upon these findings, caution should be used when consuming nonsteroidal anti-inflammatory drugs during ultra distance events.

Key words

exercise - drugs - antioxidant potential - lipid oxidation - protein oxidation

References

• 1 Ashton T, Young I S, Davison G W, Rowlands C C, McEneny J, Van B C, Jones E, Peters J R, Jackson S K. Exercise-induced endotoxemia: the effect of ascorbic acid supplementation.  Free Radic Biol Med. 2003;  35 284-291
• 2 Barlas P, Craig J A, Robinson J, Walsh D M, Baxter G D, Allen J M. Managing delayed-onset muscle soreness: lack of effect of selected oral systemic analgesics.  Arch Phys Med Rehabil. 2000;  81 966-972
• 3 Basu S, Eriksson M. Oxidative injury and survival during endotoxemia.  FEBS Lett. 1998;  438 159-160
• 4 Beaton L J, Allan D A, Tarnopolsky M A, Tiidus P M, Phillips S M. Contraction-induced muscle damage is unaffected by vitamin E supplementation.  Med Sci Sports Exerc. 2002;  34 798-805
• 5 Benzie I FF, Strain J J. The ferric reducing ability of plasma (FRAP) as a measure of “antioxidant power”: the FRAP assay.  Anal Biochem. 1996;  239 70-76
• 6 Bloomer R J, Goldfarb A H, Wideman L, McKenzie M J, Consitt L A. Effects of acute aerobic and anaerobic exercise on blood markers of oxidative stress.  J Strength Cond Res. 2005;  19 276-285
• 7 Boelsterli U A. Diclofenac-induced liver injury: a paradigm of idiosyncratic drug toxicity.  Toxicol Appl Pharmacol. 2003;  192 307-322
• 8 Bosenberg A T, Brock-Utne J G, Gaffin S L, Wells M T, Blake G T. Strenuous exercise causes systemic endotoxemia.  J Appl Physiol. 1988;  65 106-108
• 9 Bourgeois J, MacDougall D, MacDonald J, Tarnopolsky M. Naproxen does not alter indices of muscle damage in resistance-exercise trained men.  Med Sci Sports Exerc. 1999;  31 4-9
• 10 Child R B, Wilkinson D M, Fallowfield J L, Donnelly A E. Elevated serum antioxidant capacity and plasma malondialdehyde concentration in response to a simulated half-marathon run.  Med Sci Sports Exerc. 1998;  30 1603-1607
• 11 Criswell D, Powers S, Dodd S, Lawler J, Edwards W, Renshler K, Grinton S. High intensity training-induced changes in skeletal muscle antioxidant enzyme activity.  Med Sci Sports Exerc. 1993;  25 1135-1140
• 12 Deskur E, Przywarska I, Dylewicz P, Szczesniak L, Rychlewski T, Wilk M, Wysocki H. Exercise-induced increase in hydrogen peroxide plasma levels is diminished by endurance training after myocardial infarction.  Int J Cardiol. 1998;  67 219-224
• 13 Dill D B, Costill D L. Calculation of percentage changes in volumes of blood, plasma, and red cells in dehydration.  J Appl Physiol. 1974;  37 247-248
• 14 Dogra G, Ward N, Croft K D, Mori T A, Barrett P H, Herrmann S E, Irish A B, Watts G F. Oxidant stress in nephrotic syndrome: comparison of F(2)-isoprostanes and plasma antioxidant potential.  Nephrol Dial Transplant. 2001;  16 1626-1630
• 15 Donnelly A E, McCormick K, Maughan R J, Whiting P H, Clarkson P M. Effects of a non-steroidal anti-inflammatory drug on delayed onset muscle soreness and indices of damage.  Br J Sports Med. 1988;  22 35-38
• 16 Goldfarb A H, Patrick S W, Bryer S, You T. Vitamin C supplementation affects oxidative-stress blood markers in response to a 30-minute run at 75 % V·O_2max.  Int J Sport Nutr Exerc Metab. 2005;  15 279-290
• 17 Goto S, Nakamura A, Radak Z, Nakamoto H, Takahashi R, Yasuda K, Sakurai Y, Ishii N. Carbonylated proteins in aging and exercise: immunoblot approaches.  Mech Ageing Dev. 1999;  107 245-253
• 18 Hinchcliff K W, Reinhart G A, DiSilvestro R, Reynolds A, Blostein-Fujii A, Swenson R A. Oxidant stress in sled dogs subjected to repetitive endurance exercise.  Am J Vet Res. 2000;  61 512-517
• 19 Inayama T, Kumagai Y, Sakane M, Saito M, Matsuda M. Plasma protein-bound sulfhydryl group oxidation in humans following a full marathon race.  Life Sci. 1996;  59 573-578
• 20 Ji L L. Exercise at old age: does it increase or alleviate oxidative stress?.  Ann NY Acad Sci. 2001;  928 236-247
• 21 Koller A, Mair J, Schobersberger W, Wohlfarter T, Haid C, Mayr M, Villiger B, Frey W, Puschendorf B. Effects of prolonged strenuous endurance exercise on plasma myosin heavy chain fragments and other muscular proteins. Cycling vs. running.  J Sports Med Phys Fitness. 1998;  38 10-17
• 22 Lee J, Goldfarb A H, Rescino M H, Hegde S, Patrick S, Apperson K. Eccentric exercise effect on blood oxidative-stress markers and delayed onset of muscle soreness.  Med Sci Sports Exerc. 2002;  34 443-448
• 23 Leeuwenburgh C, Hansen P A, Holloszy J O, Heinecke J W. Oxidized amino acids in the urine of aging rats: potential markers for assessing oxidative stress in vivo.  Am J Physiol. 1999;  276 R128-R135
• 24 Mastaloudis A, Morrow J D, Hopkins D W, Devaraj S, Traber M G. Antioxidant supplementation prevents exercise-induced lipid peroxidation, but not inflammation, in ultramarathon runners.  Free Radic Biol Med. 2004;  36 1329-1341
• 25 McAnulty S R, McAnulty L, Pascoe D D, Gropper S S, Keith R E, Morrow J D, Gladden L B. Hyperthermia increases exercise-induced oxidative stress.  Int J Sports Med. 2005;  26 188-192
• 26 McAnulty S R, McAnulty L S, Nieman D C, Morrow J D, Shooter L A, Holmes S, Heward C, Henson D A. Effect of alpha-tocopherol supplementation on plasma homocysteine and oxidative stress in highly trained athletes before and after exhaustive exercise.  J Nutr Biochem. 2005;  16 530-537
• 27 Morrow J D. The isoprostanes: their quantification as an index of oxidant stress status in vivo.  Drug Metab Rev. 2000;  32 377-385
• 28 Morrow J D, Roberts L J. Mass spectrometric quantification of F₂-isoprostanes in biological fluids and tissues as measure of oxidant stress.  Methods Enzymol. 1999;  300 3-12
• 29 Morrow J D, Roberts L J. The isoprostanes: unique bioactive products of lipid peroxidation.  Prog Lipid Res. 1997;  36 1-21
• 30 Nieman D C, Dumke C I, Henson D A, McAnulty S R, McAnulty L S, Lind R H, Morrow J D. Immune and oxidative changes during and following the Western States Endurance Run.  Int J Sports Med. 2003;  24 541-547
• 31 Nieman D C, Dumke C L, Henson D A, McAnulty S R, Gross S J, Lind R H. Muscle damage is linked to cytokine changes following a 160-km race.  Brain Behav Immun. 2005;  19 398-403
• 32 Nourooz-Zadeh J, Tajaddini-Sarmadi J, Ling E, Wolff S. Low-density lipoprotein is the major carrier of lipid hydroperoxides in plasma.  Bio J. 1996;  313 781-786
• 33 Nyyssonen K, Porkkala-Sarataho E, Kaikkonen J, Salonen J T. Ascorbate and urate are the strongest determinants of plasma antioxidative capacity and serum lipid resistance to oxidation in Finnish men.  Atherosclerosis. 1997;  130 223-233
• 34 Saxton J M, Donnelly A E, Roper H P. Indices of free-radical mediated damage following maximum voluntary eccentric and concentric muscular work.  Eur J Appl Physiol. 1994;  68 189-193
• 35 Tokmakidis S P, Kokkinidis E A, Smilios I, Douda H. The effects of ibuprofen on delayed muscle soreness and muscular performance after eccentric exercise.  J Strength Cond Res. 2003;  17 53-59
• 36 Vider J, Lehtmaa J, Kullisaar T, Vihalemm T, Zilmer K, Kairane C, Landor A, Karu T, Zilmer M. Acute immune response in respect to exercise-induced oxidative stress.  Pathophysiology. 2001;  7 263-270
• 37 Warren J A, Jenkins R R, Packer L, Witt E H, Armstrong R B. Elevated muscle vitamin E does not attenuate eccentric exercise-induced muscle injury.  J Appl Physiol. 1992;  72 2168-2175