Antioxidant Flavonoids and Isoflavonoids from Rhizomes of Iris pseudopumila

Abstract

Chromatographic separation of a methanolic extract from Iris pseudopumila rhizomes led to the isolation of two new natural compounds, irilone 4′-O-[β-D-glucopyranosyl-(1→6)-β-D-glucopyranoside] and isoscutellarein 6-C-β-D-glucopyranoside, along with 9 isoflavonoids and 4 flavonoids. Their structures were determined on the basis of spectroscopic evidence. Isolated compounds were evaluated for their antioxidant activity using the luminol-dependent chemiluminescence assay, with quercetin as a positive control. Irilone 4′-O-β-D-glucopyranoside and kaempferol 3-O-[α-L-rhamnopyranosyl-(1→6)-β-D-glucopyranoside] showed significant antioxidant activity.

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