Brefeldin A Action and Recovery in Chlamydomonas are Rapid and Involve Fusion and Fission of Golgi Cisternae

E. Hummel; R. Schmickl; G. Hinz; S. Hillmer; D. G. Robinson

doi:10.1055/s-2006-924759

Plant Biology, Table of Contents

Plant Biol (Stuttg) 2007; 9(4): 489-501
DOI: 10.1055/s-2006-924759

Research Paper

Georg Thieme Verlag Stuttgart KG · New York

Brefeldin A Action and Recovery in Chlamydomonas are Rapid and Involve Fusion and Fission of Golgi Cisternae

E. Hummel[*] ¹ , R. Schmickl[*] ¹ , G. Hinz¹ , S. Hillmer¹ , D. G. Robinson¹

¹Department of Cell Biology, Heidelberg Institute for Plant Sciences (HIP), University of Heidelberg, Im Neuenheimer Feld 230, 69120 Heidelberg, Germany

Abstract

Chlamydomonas noctigama has a non-motile Golgi apparatus consisting of several Golgi stacks adjacent to transitional ER. These domains are characterized by vesicle-budding profiles and the lack of ribosomes on the side of the ER proximal to the Golgi stacks. Immunogold labelling confirms the presence of COPI-proteins at the periphery of the Golgi stacks, and COPII-proteins at the ER-Golgi interface. After addition of BFA (10 µg/ml) a marked increase in the number of vesicular profiles lying between the ER and the Golgi stacks is seen. Serial sections of cells do not provide any evidence for the existence of tubular connections between the ER and the Golgi stacks, supporting the notion that COPI- but not COPII-vesicle production is affected by BFA. The fusion of COPII-vesicles at the cis-Golgi apparatus apparently requires the presence of retrograde COPI-vesicles. After 15 min the cisternae of neighbouring Golgi stacks begin to fuse forming “mega-Golgis”, which gradually curl before fragmenting into clusters of vesicles and tubules. These are surrounded by the transitional ER on which vesicle-budding profiles are still occasionally visible. Golgi remnants continue to survive for several hours and do not completely disappear. Washing out BFA leads to a very rapid reassembly of Golgi cisternae. At first, clusters of vesicles are seen adjacent to transitional ER, then “mini Golgis” are seen whose cisternae grow in length and number to produce “mega Golgis”. These structures then divide by vertical fission to produce Golgi stacks of normal size and morphology roughly 60 min after drug wash-out.

Key words

Brefeldin A - Chlamydomonas - COP-vesicles - Golgi fission - Golgi fusion.

Full Text

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1 These authors contributed equally to this paper

D. G. Robinson

Department of Cell Biology
Heidelberg Institute for Plant Sciences (HIP)
University of Heidelberg

Im Neuenheimer Feld 230

69120 Heidelberg

Germany

Email: david.robinson@urz.uni-heidelberg.de

Editor: S. M. Wick