Hybrid Lethality in Interspecific F₁ Hybrid Nicotiana gossei × N. tabacum Involves a MAP-Kinases Signalling Cascade

Abstract

A cultured cell line, GTH4 (Nicotiana gossei Domin × N. tabacum L.), which exhibits hybrid lethality, died at 26 °C, but not at 37 °C. Pharmacological experiments using inhibitors of protein phosphatases and protein kinases indicated the involvement of a protein kinase signalling pathway in the cell death process. Immunoblot analysis revealed that salicylic acid-induced protein kinase (SIPK) was phosphorylated soon after the shift in temperature from 37 °C to 26 °C. Cultured cells of the hybrid of N. gossei × transgenic N. tabacum harboring a steroid (dexamethasone; DEX)-inducible NtMEK2^DD or NtMEK2^KR, constitutively active and inactive forms of NtMEK2, respectively, were established. Induction of NtMEK2^DD by DEX in the hybrid cells induced the activation of SIPK, the generation of hydrogen peroxide (H₂O₂), and cell death at 37 °C. The activation of SIPK, generation of H₂O₂, and cell death at 26 °C were compromised by DEX treatment in hybrid cells harbouring NtMEK2^KR. This study provides evidence for the involvement of MAPK signalling in the regulation of cell death in hybrids.

Key words

Hybrid lethality - MAPK signalling - Nicotiana - oxidative burst - programmed cell death - SIPK - WIPK.

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Editor: J. Cullimore