Anti-Inflammatory Effect of Kamebakaurin in in vivo Animal Models

Abstract

We have identified kamebakaurin as an inhibitor of NF-κB and elucidated its molecular mechanism as a specific inhibitor in the DNA-binding activity of the p50 subunit of NF-κB. Here, we describe its anti-inflammatory activity in in vitro and in vivo models. Kamebakaurin dose-dependently inhibited not only the expression of inflammatory NF-κB target genes such as iNOS, COX-2, and TNF-α, but also the production of PGE₂ and TNF-α in LPS-stimulated RAW264.7 cells. Moreover, in an air pouch model of inflammation, it suppressed the recruitment of neutrophils, production of TNF-α as well as PGE₂ in the pouch exudates induced by carrageenan. In addition, kamebakaurin dose-dependently suppressed the inflammation in an adjuvant arthritis model. Oral administration of 20 mg/kg kamebakaurin resulted in the 75 % decrease of paw volume. Taken together, kamebakaurin, a specific inhibitor of DNA-binding activity of the p50 subunit, is a valuable candidate for the intervention in NF-κB-dependent pathological conditions such as inflammation.

Key words

Diterpene - kamebakaurin - NF-κB - p50 - anti-inflammation - air pouch - adjuvant arthritis

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