Mutation Analysis of Leukemia Inhibitory Factor-Receptor (LIF-R) in ACTH-Secreting Pituitary Adenomas

Abstract

Numerous cytokines and particularly leukemia inhibitory factor (LIF) are involved in the differentiation, proliferation, and secretory function of ACTH-producing pituitary cells. LIF and its receptor LIF-R are abundantly expressed in normal pituitaries and in ACTH producing adenomas. The present study was performed to evaluate the possible role of LIF-R in the pathogenesis of ACTH-secreting pituitary adenomas.

To test the hypothesis that a possible mutation of LIF-R might be involved in pathogenesis of Cushing's disease we analyzed ACTH-producing pituitary adenomas as well as inactive pituitary adenomas by RT-PCR and direct cycle sequencing. No mutations have been found in the adenomas investigated. We thus conclude that mutations in LIF-R are an unlikely cause for the development of Cushing's disease.

Key words

Leukemia inhibitory factor-receptor - pituitary adenoma - mutation analysis

References

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