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Exp Clin Endocrinol Diabetes 2004; 112(8): 435-439
DOI: 10.1055/s-2004-821189
Article

J. A. Barth Verlag in Georg Thieme Verlag KG Stuttgart · New York

Interleukin-6 (IL-6) and IGF-IGFBP System in Children and Adolescents with Type 1 Diabetes Mellitus[*]

A. Wędrychowicz1 , H. Dziatkowiak1 , K. Sztefko2 , A. Wędrychowicz3
  • 1Department of Pediatric Endocrinology, Polish-American Children's Hospital, Jagiellonian University, Kraków, Poland
  • 2Department of Clinical Biochemistry, Polish-American Children's Hospital, Jagiellonian University, Kraków, Poland
  • 3Department of Pediatrics, Gastroenterology and Nutrition, Polish-American Children's Hospital, Jagiellonian University, Kraków, Poland
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Publication Date:
16 September 2004 (online)

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Abstract

Background: Some studies suggest that IL-6 is connected with glucose metabolism and insulin action, so like IGF-IGFBPs system it could play the role in diabetes etiopathogenesis.

Aim: The aim of the study was therefore to test the hypothesis that in children and adolescents IL-6 is of importance for the etiopathogenesis of diabetes mellitus type 1 and that IL-6 is connected with carbohydrate metabolism and IGF-IGFBPs action.

Methods: There were 49 patients with type 1 diabetes: 10 persons at onset diabetes and 39 with disease lasted at least 1 year and 33 age-matched healthy children included into the study. Serum IGF-I concentrations were measured by RIA; IGFBP-1 and IGFBP-2 by IRMA and IL-6 concentrations using quantitative ELISA immunoassays. HbA1c was measured by HPLC method. Multiple regression, ANOVA, and Student's t-test were used for statistical analysis.

Results: IL-6 and IGF-I, IGFBP-1 and IGFBP-2 levels did not differ statistically significant between diabetic patients and controls. IL-6 concentrations were statistically higher at onset diabetes than in diabetic patients with long-term disease. IL-6 did not correlate with IGF-I and its binding proteins - 1 and - 2 in examined groups. At onset of disease IL-6 correlated with insulin requirement. There were not found correlations between IL-6 and HbA1c in diabetic groups. IGF-I positively correlated with age and growth in both groups and with IGFBP-2 in diabetic patients. IGFBP-2 correlated negatively with BMI in all examined groups.

Conclusions: IL-6 might play a significant role in type 1 diabetes mellitus etiopathogenesis. It seems that IL-6 does not regulate IGF-IGFBP system in diabetic children and adolescents or IL-6 maybe cooperate with IGF-I, but their significant dependence on insulin action influences their mutual relationships.

Key words

Type 1 diabetes mellitus - diabetes onset - IL-6 - IGF-I - IGFBPs

1 A preliminary version of this study was presented at ISPAD 2001, Sienna, Italy

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1 A preliminary version of this study was presented at ISPAD 2001, Sienna, Italy

M. D., Ph.D. Anna Wędrychowicz

Polish-American Children's Hospital

ul. Wielicka 265

30-663 Kraków

Poland

Phone: + 48126581277

Fax: + 48 1 26 58 10 05

Email: miniedzw@cyf-kr.edu.pl