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Plant Biol (Stuttg) 2004; 6(4): 402-407
DOI: 10.1055/s-2004-817959
Original Paper

Georg Thieme Verlag Stuttgart KG · New York

The Cell Wall-Modifying Xyloglucan Endotransglycosylase/Hydrolase LeXTH1 is Expressed during the Defence Reaction of Tomato against the Plant Parasite Cuscuta reflexa

M. Albert1 , M. Werner1 , P. Proksch2 , S. C. Fry3 , R. Kaldenhoff1
  • 1TU Darmstadt, Botanisches Institut, Darmstadt, Germany
  • 2Heinrich-Heine-Universität Düsseldorf, Institut für Pharmazeutische Biologie, Universitätsstr. 1, 40225 Düsseldorf, Germany
  • 3The Edinburgh Cell Wall Group, Institute of Cell and Molecular Biology, The University of Edinburgh, Daniel Rutherford Building, The King's Buildings, Edinburgh EH9 3JH, UK
Further Information

Publication History

Publication Date:
12 July 2004 (online)

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Abstract

A suppressive subtractive hybridization technique was used to identify genes, which were induced during the early phases of the interaction between dodder (Cuscuta reflexa), a phanerogamic parasite, and its incompatible host plant tomato. One of the identified genes encodes a tomato xyloglucan endotransglycosylase/hydrolase (XTH) - an enzyme involved in cell wall elongation and restructuring. The corresponding LeXTH1 mRNA accumulated 6 h after attachment of the parasite. In contrast, wounding did not influence the expression level. Subsequent to LeXTH1 mRNA accumulation, an increase in XTH activity at the infection sites as well as in adjacent tissues was observed. The effect of IAA on LeXTH1 expression was analyzed because the concentration of this phytohormone is known to increase in the tomato tissue during the interaction with the parasite. LeXTH1 mRNA accumulation was in fact induced by external application of auxin. However, in the auxin-insensitive tomato mutant diageotropica, Cuscuta induced LeXTH1-mRNA accumulated with a time course similar to wild type tomato. Thus, auxin appears not to be an essential signal for infection-induced LeXTH1 activation. Our data suggest a role for xyloglucan transglycosylation in defence reactions associated with the incompatible tomato-Cuscuta interaction.

Key words

Plant interaction - Cuscuta reflexa - tomato - xyloglucan endotransglycosylase/hydrolase.

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R. Kaldenhoff

Botanisches Institut
TU Darmstadt

Schnittspahnstraße 10

64287 Darmstadt

Germany

Email: kaldenhoff@bio.tu-darmstadt.de

Section Editor: G. Thiel