Planta Med 2003; 69(12): 1147-1149
DOI: 10.1055/s-2003-818007
Letter
© Georg Thieme Verlag Stuttgart · New York

Inhibitory Effects of Manassantin A and B Isolated from the Roots of Saururus chinensis on PMA-Induced ICAM-1 Expression

Mun-Chual Rho1 , Oh Eok Kwon1 , Koanhoi Kim1 , Seung Woong Lee1 , Mi Yeon Chung1 , Young Ho Kim2 , Masahiko Hayashi3 , Hyun Sun Lee1 , Young-Kook Kim1
  • 1Laboratory of Lipid Metabolism, Korea Research Institute of Bioscience and Biotechnology, Taejon, Korea
  • 2College of Pharmacy, Chungnam National University, Taejon, Korea
  • 3Kitasato Institute for Life Sciences, Tokyo, Japan
This research was supported by a grant (PDM0020213) from Plant Diversity Research Center of 21st Century Frontier Research Program funded by Ministry of Science and Technology of Korean government
Further Information

Publication History

Received: May 22, 2003

Accepted: August 23, 2003

Publication Date:
29 January 2004 (online)

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Abstract

Cell adhesion inhibitors were isolated from the methanol extract of Saururus chinensis roots by bioactivity-guided fractionation. The active compounds were identified as manassantin A (1) and B (2), dineolignan compounds. Compounds 1 and 2 inhibited PMA-induced ICAM-1/LFA-1-mediated homotypic aggregation of the HL-60 cells without cytotoxicity with MIC values of 1.0 and 5.5 nM, respectively. Even though 1 and 2 did not affect the adhesion of ICAM-1 to LFA-1, these compounds inhibited PMA-induced ICAM-1 expression in HL-60 cells in a dose-dependent fashion. These results suggest that 1 and 2 inhibit cell aggregation through down-regulation of ICAM-1 expression.

References

Dr. Hyun Sun Lee

Laboratory of Lipid Metabolism

Korea Research Institute of Bioscience and Biotechnology

52 Oun-dong

Yusong-gu

Taejon 305-333

Korea

Phone: +82-42-860-4314

Fax: +82-42-861-2675

Email: leehs@kribb.re.kr