Planta Med 2003; 69(9): 871-874
DOI: 10.1055/s-2003-43203
Letter
© Georg Thieme Verlag Stuttgart · New York

Chemical Composition and Antifungal Activity of the Essential Oil of Origanum virens on Candida Species

L. R. Salgueiro1 , C. Cavaleiro1 , E. Pinto2 , C. Pina-Vaz3 , 5 , A. G. Rodrigues3 , 5 , A. Palmeira2 , C. Tavares3 , S. Costa-de-Oliveira3 , M. J. Gonçalves1 , J. Martinez-de-Oliveira4
  • 1Lab. de Farmacognosia, Fac. de Farmácia/CEF, Universidade de Coimbra, Coimbra, Portugal
  • 2Serviço de Microbiologia/CEQOFF, Fac. de Farmácia, Universidade do Porto, Porto, Portugal
  • 3Serviço de Microbiologia, Fac. de Medicina, Universidade do Porto, Porto, Portugal
  • 4Dep. de Ginecologia e Obstetricia, Fac. de Medicina, Universidade do Porto, Porto, Portugal
  • 5IPATIMUP, Porto, Portugal
Further Information

Publication History

Received: February 13, 2003

Accepted: May 10, 2003

Publication Date:
04 November 2003 (online)

Abstract

The composition and the antifungal activity of the essential oil of Origanum virens on Candida species were studied. The essential oil was obtained from the aerial parts of the plant by hydrodistillation and analyzed by GC and GC-MS. The oil was characterized by its high content of carvacrol (68.1 %) and its biogenetic precursors, γ-terpinene (9.9 %) and p-cymene (4.5 %). The minimal inhibitory concentration (MIC) and the minimal lethal concentration (MLC) were used to evaluate the antifungal activity against Candida strains (7 clinical isolates and 3 ATCC type strains). The inhibition of germ tube formation and flow cytometry, using the fluorescent probe propidium iodide (PI), were used to evaluate their mechanisms of action. MIC and MLC values were similar for most tested strains, ranging from 0.16 to 0.32 μL/mL. Concentrations lower than MIC values strongly prevent germ tube formation. The fungicidal effect is primarily due to an extensive lesion of the membrane.

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Prof. Dr. Lígia Salgueiro

Laboratório de Farmacognosia

Faculdade de Farmácia de Coimbra

3000 Coimbra

Portugal

Fax: +351-239827126

Email: ligia@ff.uc.pt