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J Reconstr Microsurg 2003; 19(1): 041-048
DOI: 10.1055/s-2003-37190
Copyright © 2002 by Thieme Medical Publishers, Inc., 333 Seventh Avenue, New York, NY 10001, USA. Tel.: +1(212) 584-4662

Functional Recovery and Histomorphometric Assessment Following Tibial Nerve Injury in the Mouse

Laura T. George, Terence M. Myckatyn, John N. Jensen, Daniel A. Hunter, Susan E. Mackinnon
  • Division of Plastic and Reconstructive Surgery, Washington University School of Medicine, St. Louis, MO
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Publication History

Publication Date:
12 February 2003 (online)

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ABSTRACT

Longitudinal studies have established that functional recovery following sciatic nerve injury can be evaluated in the mouse. Injury to the tibial nerve offers several advantages to sciatic nerve injury, including improved lower extremity sensation and end-organ reinnervation. Functional recovery following tibial nerve crush injury was studied in 55 C3H mice randomized into five groups harvested for histomorphometric evaluation from either normal nerves or 2, 3, 4, or 6 weeks postoperatively. Walking tracks were obtained preoperatively, and at regular intervals postoperatively, and foot print lengths measured. Significant normalization of print length occurred 14 days postoperatively, and complete recovery was noted 28 days postoperatively. Significant histomorphologic evidence of neuroregeneration was detected between 2 and 4 weeks postoperatively. Injury to the tibial nerve is a viable alternative to the sciatic nerve for studying neural regeneration in mice, and the print length factor can be used to monitor functional recovery in this model.

KEYWORD

Tibial nerve crush - axonotmesis - walking track - mouse

REFERENCES

  • 1 Dellon A L, Mackinnon S E. Sciatic nerve regeneration in the rat.  Validity of walking track assessment in the presence of chronic contractures. Microsurgery . 1989;  10 220-225
    PubMedGoogle Scholar
  • 2 Feng F Y, Ogden M A, Myckatyn T M. FK506 rescues peripheral nerve allografts in acute rejection.  J Neurotrauma . 2001;  18 217-229
    CrossrefPubMedGoogle Scholar
  • 3 Ogden M A, Feng F Y, Myckatyn T M. Safe injection of cultured Schwann cells into peripheral nerve allografts [In Process Citation].  Microsurgery . 2000;  20 314-323
    CrossrefPubMedGoogle Scholar
  • 4 Calvert G T, Doolabh V B, Grand A G, Hunter D A, Mackinnon S E. Rat-strain differences in recovery following peripheral-nerve allotransplantation.  J Reconstr Microsurg . 2001;  17 185-191
    Article in Thieme ConnectPubMedGoogle Scholar
  • 5 de Medinaceli L, Freed W J, Wyatt R J. An index of the functional condition of rat sciatic nerve based on measurements made from walking tracks.  Exp Neurol . 1982;  77 634-643
    CrossrefPubMedGoogle Scholar
  • 6 Bain J R, Mackinnon S E, Hunter D A. Functional evaluation of complete sciatic, peroneal, and posterior tibial nerve lesions in the rat.  Plast Reconstr Surg . 1989;  83 129-138
    CrossrefPubMedGoogle Scholar
  • 7 Hare G M, Evans P J, Mackinnon S E. Walking track analysis: a long-term assessment of peripheral nerve recovery [see comments].  Plast Reconstr Surg . 1992;  89 251-258
    CrossrefPubMedGoogle Scholar
  • 8 Hare G M, Evans P J, Mackinnon S E. Walking track analysis: utilization of individual footprint parameters.  Ann Plast Surg . 1993;  30 147-153
    CrossrefPubMedGoogle Scholar
  • 9 Brown C J, Evans P J, Mackinnon S E. Inter- and intraobserver reliability of walking-track analysis used to assess sciatic nerve function in rats.  Microsurgery . 1991;  12 76-79
    CrossrefPubMedGoogle Scholar
  • 10 Munro C A, Szalai J P, Mackinnon S E, Midha R. Lack of association between outcome measures of nerve regeneration.  Muscle Nerve . 1998;  21 1095-1097
    CrossrefPubMedGoogle Scholar
  • 11 Shenaq J M, Shenaq S M, Spira M. Reliability of sciatic function index in assessing nerve regeneration across a 1 cm gap.  Microsurgery . 1989;  10 214-219
    PubMedGoogle Scholar
  • 12 Kanaya F, Firrell J C, Breidenbach W C. Sciatic function index, nerve conduction tests, muscle contraction, and axon morphometry as indicators of regeneration.  Plast Reconstr Surg . 1996;  98 1264-1272
    CrossrefPubMedGoogle Scholar
  • 13 Levi A D, Bunge R P. Studies of myelin formation after transplantation of human Schwann cells into the severe combined immunodeficient mouse.  Exp Neurol . 1994;  130 41-52
    CrossrefPubMedGoogle Scholar
  • 14 Osawa T, Idle C, Tohyama K. Nerve regeneration through allogeneic nerve grafts in mice.  Archivum Histologicum Japonicum-Nippon Soshikigaku Kiroku . 1986;  49 69-81
    PubMedGoogle Scholar
  • 15 Yao M, Moir M S, Wang M Z, To M P, Terris DJ Peripheral nerve regeneration in CNTF knockout m ice. Laryngoscope .  1999;  109 1263-1268
    PubMedGoogle Scholar
  • 16 Inserra M M, Yao M, Murray R, Terris D J. Peripheral nerve regeneration in interleukin 6-deficient mice.  Arch Otolaryngol Head Neck Surg . 2000;  126 1112-1116
    CrossrefPubMedGoogle Scholar
  • 17 Rodriguez F J, Verdu E, Ceballos D, Navarro X. Nerve guides seeded with autologous Schwann cells improve nerve regeneration.  Exp Neurol . 2000;  161 571-584
    CrossrefPubMedGoogle Scholar
  • 18 Gold B G. FK506 and the role of the immunophilin FKBP-52 in nerve regeneration.  Drug Metab Rev . 1999;  31 649-663
    CrossrefPubMedGoogle Scholar
  • 19 Levy D, Kubes P, Zochodne D W. Delayed peripheral nerve degeneration, regeneration, and pain in mice lacking inducible nitric oxide synthase.  J Neuropathol Exp Neurol . 2001;  60 411-421
    PubMedGoogle Scholar
  • 20 Xian C J, Li L, Deng Y S, Zhao S P, Zhou X F. Lack of effects of transforming growth factor-alpha gene knockout on peripheral nerve regeneration may result from compensatory mechanisms.  Exp Neurol . 2001;  172 182-188
    CrossrefPubMedGoogle Scholar
  • 21 Ferri C C, Moore F A, Bisby M A. Effects of facial nerve injury on mouse motoneurons lacking the p75 low- affinity neurotrophin receptor.  J Neurobiol . 1998;  34 1-9
    CrossrefPubMedGoogle Scholar
  • 22 Strittmatter S M, Fankhauser C, Huang P L. Neuronal pathfinding is abnormal in mice lacking the neuronal growth cone protein GAP-43.  Cell . 1995;  80 445-452
    CrossrefPubMedGoogle Scholar
  • 23 Myckatyn T M, Ellis R A, Grand A G. The effects of Rapamycin in murine peripheral nerve isografts and allografts.  Plast Reconstr Surg . 2002;  109(7) 2405-2417
    PubMedGoogle Scholar
  • 24 Tung T HH, Mohanakumar T, Mackinnon S E. A subcutaneous heterotopic limb transplantation model in the mouse for prolonged allograft survivals.  Microsurgery . 2001;  21 298-305
    CrossrefPubMedGoogle Scholar
  • 25 Inserra M M, Bloch D A, Terris D J. Functional indices for sciatic, peroneal, and posterior tibial nerve lesions in the mouse.  Microsurgery . 1998;  18 119-124
    CrossrefPubMedGoogle Scholar
  • 26 Yao M, Inserra M M, Duh M J, Terris D J. A longitudinal, functional study of peripheral nerve recovery in the mouse.  Laryngoscope . 1998;  108 1141-1145
    PubMedGoogle Scholar
  • 27 Grand A G, Myckatyn T M, Mackinnon S E, Hunter D A. Cold preservation of nerve allografts and immunosuppression with FK506 in mice.  J Neurosurg . 2002;  95(5) 924-932
    PubMedGoogle Scholar
  • 28 Bridge P M, Ball D J, Mackinnon S E. Nerve crush injuries-a model for axonotmesis.  Exp Neurol . 1994;  127 284-290
    CrossrefPubMedGoogle Scholar
  • 29 Mackinnon S E, Dellon A L, O'Brien J P. Changes in nerve fiber numbers distal to a nerve repair in the rat sciatic nerve model.  Muscle Nerve . 1991;  14 1116
    Google Scholar
  • 30 Fu S Y, Gordon T. The cellular and molecular basis of peripheral nerve regeneration.  Mol Neurobiol . 1997;  14 67-116
    CrossrefPubMedGoogle Scholar
  • 31 Brown M C, Lunn E R, Perry V H. Consequences of slow Wallerian degeneration for regenerating motor and sensory axons.  J Neurobiol . 1992;  23 521-536
    CrossrefPubMedGoogle Scholar
  • 32 Dailey A T, Avellino A M, Benthem L, Silver J, Kliot M. Complement depletion reduces macrophage infiltration and activation during Wallerian degeneration and axonal regeneration.  J Neurosci . 1998;  18 6713-6722
    PubMedGoogle Scholar
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