Hepatoprotective and Free Radical Scavenging Activities of Prenylflavonoids, Coumarin, and Stilbene from Morus alba

Abstract

Chemical investigation of the EtOH extract of Morus alba L. (Moraceae), as guided by free radical scavenging activity, furnished 5,7-dihydroxycoumarin 7-methyl ether (1), two prenylflavones, cudraflavone B (2) and cudraflavone C (3), and oxyresveratrol (4). Compounds 1 and 4 showed superoxide scavenging effects with the IC₅₀ values of 19.1 ± 3.6 and 3.81 ± 0.5 μM, respectively. Compound 4 exhibited a DPPH free radical scavenging effect (IC₅₀ = 23.4 ± 1.5 μM). Compounds 2 and 4 showed hepatoprotective effects with EC₅₀ values of 10.3 ± 0.42 and 32.3 ± 2.62 μM, respectively, on tacrine-induced cytotoxicity in human liver-derived Hep G2 cells.

References

• 1 Bast A, Haenen G, Doelman C. Oxidants and antioxidants: state of the art.  The American Journal of Medicine. 1991;  91 Suppl 3C 2-13
• 2 Bulkley G B. Free radicals and other reactive oxygen metabolites: clinical relevance and the therapeutic efficacity of antioxidant therapy.  Surgery. 1993;  113 479-83
• 3 Halliwell B. Free radicals, antioxidants and human disease: curiosity, cause or consequence?.  Lancet. 1994;  344 721-4
• 4 Kappus H. Lipid peroxidation: mechanisms, enzymology and biological relevance. In: Sies H, editor Oxidative Stress. Academic Press London; 1985: 273-310
• 5 Kappus H. Oxidative stress in chemical toxicity.  Archives of Toxicology. 1987;  60 144-9
• 6 Zhu Y P. Chinese Materia Medica: Chemistry, Pharmacology and Applications. Amsterdam; Harwood Academic Publishers 1998: 273-4
• 7 Fujimoto T, Hano Y, Nomura T, Uzawa J. Components of root bark of Cudrania tricuspidata 2. Structures of two new isoprenylated flavones, cudraflavones A and B.  Planta Medica. 1984;  50 161-3
• 8 Hano Y, Matsumoto Y, Shinohara K, Sun J Y, Nomura T. Cudraflavones C and D, two new prenylflavones from the root bark of Cudrania tricuspidata (Carr.) Bur.  Heterocycles. 1990;  31 1339-44
• 9 Song E K, Cho H, Kim J S, Kim N Y, An N H, Kim J A, Lee S H, Kim Y C. Diarylheptanoids with free radical scavenging and hepatoprotective activity in vitro from Curcuma longa .  Planta Medica. 2001;  67 876-7