Plant Biol (Stuttg) 2001; 3(5): 489-492
DOI: 10.1055/s-2001-17726
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GeorgThieme Verlag Stuttgart ·New York

Microinjection of Guanine NucleotideAnalogues into Lily Pollen Tubes Results in Isodiametric TipExpansion

M. Eliáš1 , F. Cvrčková1 , G. Obermeyer2 , V. žársky1,3
  • 1 Faculty of Sciences, Charles University,Prague, Czech Republic
  • 2 Inst. of Plant Physiology,Paris-Lodron University, Salzburg, Austria
  • 3 Institute ofExperimental Botany, Prague, CzechRepublic
Further Information

Publication History

November 22,2000

July 6, 2001

Publication Date:
11 October 2001 (online)

Abstract

Heterotrimeric and small G-proteins aresupposed to participate in tip growth of plant cells. Quantitative changes intip growth rate after introduction of non-hydrolysable guanine nucleotideanalogues (NA) into lily pollen tubes have been interpreted as support for thehypothesis that heterotrimeric G-proteins regulate tube elongation (Ma et al.,1999[11]). Here, we report that microinjection of guanineNA into lily pollen tubes causes loss of growth polarity, resulting inisodiametric tip swelling. Our results are compatible with current modelssuggesting an involvement of plant Rho-related small G-proteins (Rop) in themaintenance of pollen tube polarity and in tip morphogenesis.

Abbreviations

ADP-β-S: adenosine5′-O-(2-thiodiphosphate)

ATP-γ-S: adenosine5′-O-(3-thiotriphosphate)

GDP-β-S: guanosine5′-O-(2-thiodiphosphate)

GTP-γ-S: guanosine5′-O-(3-thiotriphosphate)

NA: nucleotideanalogues

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V. žársky

Institute of Experimental Botany

Rozvojová 135
CZ 160 00 Praha 6
Czech Republic

Email: zarsky@ueb.cas.cz

Section Editor: A. M. C. Emons