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DOI: 10.1055/s-2001-17726
GeorgThieme Verlag Stuttgart ·New York
Microinjection of Guanine NucleotideAnalogues into Lily Pollen Tubes Results in Isodiametric TipExpansion
Publication History
November 22,2000
July 6, 2001
Publication Date:
11 October 2001 (online)

Abstract
Heterotrimeric and small G-proteins aresupposed to participate in tip growth of plant cells. Quantitative changes intip growth rate after introduction of non-hydrolysable guanine nucleotideanalogues (NA) into lily pollen tubes have been interpreted as support for thehypothesis that heterotrimeric G-proteins regulate tube elongation (Ma et al.,1999[11]). Here, we report that microinjection of guanineNA into lily pollen tubes causes loss of growth polarity, resulting inisodiametric tip swelling. Our results are compatible with current modelssuggesting an involvement of plant Rho-related small G-proteins (Rop) in themaintenance of pollen tube polarity and in tip morphogenesis.
Abbreviations
ADP-β-S: adenosine5′-O-(2-thiodiphosphate)
ATP-γ-S: adenosine5′-O-(3-thiotriphosphate)
GDP-β-S: guanosine5′-O-(2-thiodiphosphate)
GTP-γ-S: guanosine5′-O-(3-thiotriphosphate)
NA: nucleotideanalogues
Key words
Tip growth - Liliumlongiflorum - microinjection - pollentube - G-proteins - cell polarity
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V. žársky
Institute of Experimental Botany
Rozvojová 135
CZ 160 00 Praha 6
Czech Republic
Email: zarsky@ueb.cas.cz
Section Editor: A. M. C. Emons