It has been suggested that lactate concentrations may provide a guide to an optimal
training intensity. However, lactate concentrations established during incremental
exercise in the laboratory are not always indicative of what is occurring during constant-load
exercise at the same intensity. Ideally, lactate concentrations should be measured
during a training session and immediately reported to the athlete to ensure that the
athlete is working at the desired intensity. The purpose of this investigation was,
therefore, to determine the reliability and validity of a compact, portable lactate
analyser (ACCUSPORT; Boeringer Mannheim, Castle Hill, Australia). A total of 224 capillary
blood samples were taken from athletes who took part in routine laboratory testing.
Seventy-three of these capillary blood samples were analysed in duplicate with the
Accusport for determination of intraclass, single-trial reliability. Day-to-day intraclass
reliability of the Accusport was assessed by analyzing known concentrations of aqueous
lactate solutions on seven consecutive days. The validity of the Accusport analyser
was assessed by comparing the 224 capillary blood lactate concentrations determined
on the Accusport with the lactate concentration obtained using a MICRO STAT LM3 (Analox
Instruments Ltd., London, UK). In addition, lactate parameters derived from the lactate
concentrations obtained with the two analysers were compared. The Accusport showed
high single-trial intraclass reliability (R = 0.992; Standard Error of Measurement
[SEM] = 0.3 mmol × l-1; n = 73) and high day-to-day intraclass reliability (R = 0.993; SEM = 0.4 mmol × l-1; n = 42). Despite a strong correlation between blood lactate concentrations obtained
on the two analysers (r = 0.96; n = 224) the limits of agreement were + 1.9 to - 2.2
mmol × l-1. Although the mean values for power output, HR and lactate concentration associated
with the lactate parameters were not significantly different when determined on the
Accusport or Micro Stat, some individuals did record large differences between analysis
methods. In summary, the results of this investigation have shown that lactate concentrations
can be reliably determined within a single trial and from day-to-day using the Accusport
analyser. However, for some athletes, it is not valid to compare lactate concentrations
or lactate parameters determined on the Accusport with those determined using the
Micro Stat LM3 lactate analyser.
Key words:
Micro Stat, Dmax, lactate thresholds, reliability, validity.
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D. Bishop, Ph. D.
Department of Human Movement and Exercise Science
The University of Western Australia
Crawley, WA 6009
Australia
Phone: +61 (08) 9380 7282
Fax: +61 (08) 9380 1039
Email: dbishop@cyllene.uwa.edu.au