Summary:
Recent data indicate that TGFβ3, one member of the TGFβ-isoforms, has an important
role in bone remodeling. Up to date little is known about the expression and regulation
of TGFβ3 in man. We established a highly specific ELISA for quantitative measurement
of TGFβ3 in bone and blood samples and a RT-PCR in combination with HPLC for detection
and quantification of TGFβ3 mRNA in 89 human bone samples. Levels of TGFβ3 protein
ranged between 30 and 66 pg/mg bone (mean 36,6 ± 1,03 pg/mg) and between 30 and 1910
pg/ml in serum (mean 128,9 ± 38.9 pg/ml). TGFβ3 mRNA expression as well as protein
levels in serum and in bone declined age dependently. No specific load- or site-specific
distribution of TGFβ3 mRNA expression or protein content was detected at different
sites indicating an absence of mechanical regulation. Protein levels of TGFβ3 in serum
correlated with TGFβ3 mRNA expression in bone (p = 0.0027; r = 0.49). By contrast,
TGFβ3 protein levels stored in the bone matrix were not related to TGFβ3 mRNA reflecting
the long term process of TGFβ3 deposition during bone remodeling. Notably TGFβ3 serum
levels were highly correlated with IGF-I and osteocalcin levels in serum. We conclude
that TGFβ3 in man circulates in significant amounts which appears to be representative
for TGFβ3 expression in bone tissue and may be in part derived from bone. The high
correlation of TGFβ3 with IGF-I suggests parallel systemic principles of regulation.
Key words:
HPLC - TGFβ3-ELISA - aging - quantative-PCR - bone biopsy
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Dr. med. Steffen Hering
BG-Kliniken “Bergmannsheil”
Ruhr-University of Bochum
Department of Internal Medicine
Bürkle de la Camp Platz 1
D-44789 Bochum
Germany
Phone: + 49-234-302-6400
Fax: + 49-234-302-6403
Email: steffen.hering@ruhr-uni-bochum.de