Abstract
Bioassay-directed fractionation led to the identification of four known coumarins,
osthole (1), imperatorin (2), xanthotoxin (3), and isopimpinellin (4), from the ethanolic extract of the fruit of Cnidium monnieri (L.) Cusson. In phenylephrine (PE)-precontracted endothelium-intact rabbit corpus
cavernosum, all four coumarins exhibited relaxing effect with the IC50 values for compounds 1, 2, 3, and 4 determined to be 2.14 ± 0.73, 0.85 ± 0.16, 1.24 ± 0.45, and 18.4 ± 8.10 μM, respectively.
The four compounds were identified by comparision of their physical data (EIMS, 1H- and 13C-NMR) with those from published reports.
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Prof. Chien-Chih Chen
National Research Institute of Chinese Medicine
No. 155 - 1, Sec. 2
Li Nung St.
Peitou
Taipei 11221
Taiwan
Republic of China
Email: ccchen@cma23,nricm.edu.tw
Fax: +886-2-28264276