Whole Blood Prothrombin Time Using Diluted Tissue Factor is Shortened in Spontaneous Hypertensive Rats

Machiko Munakata; Yutaka Komiyama; Midori Masuda; Hideo Kagawa; Shosaku Nomura; Shirou Fukuhara; Hakuo Takahashi

doi:10.1055/s-2000-9810

Seminars in Thrombosis and Hemostasis, Table of Contents

Semin Thromb Hemost 2000; Volume 26(Number 01): 097-100
DOI: 10.1055/s-2000-9810

Whole Blood Prothrombin Time Using Diluted Tissue Factor is Shortened in Spontaneous Hypertensive Rats

Machiko Munakata¹ , Yutaka Komiyama¹ , Midori Masuda¹ , Hideo Kagawa² , Shosaku Nomura² , Shirou Fukuhara² , Hakuo Takahashi¹

Supported in part by a grant-in-aid for scientific research from the Japanese Ministry of Education, Science and Culture.
¹Department of Clinical Sciences and Laboratory Medicine, Kansai Medical University, Moriguchi, Osaka, Japan
²The First Department of Internal Medicine, Kansai Medical University, Moriguchi, Osaka, Japan

Abstract

ABSTRACT

Clinical screening tests for blood coagulation that use plasma as samples cannot estimate the participation of platelets, leukocytes, and erythrocytes in blood coagulation system. We developed an assay to evaluate the total coagulation ability of blood and whole blood prothrombin time (WPT) using the principle of prothrombin time with the diluted-tissue factor as a trigger and a newly developed apparatus, STA, viscosity change detection system (electromagnetic clot detection). The activation of platelets by Ca ionophore shortened WPT and increased the expression of CD62P on the platelet surface. WPTs in citrated blood of spontaneous hypertensive rats (SHR) were significantly shorter than those of controls, Wistar Kyoto rats (WKY). Plasma levels of thrombin-antithrombin III (TAT) in SHR were significantly higher than those of WKY. Moreover, WPT and TAT levels were significantly correlated. Based on these results, WPT was found to be useful to estimate the activation of blood coagulation in whole blood.

KEYWORD

Prothrombin time - whole blood - tissue factor - hypertension

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References

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