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Plant Biol (Stuttg) 2000; 2(2): 161-167
DOI: 10.1055/s-2000-9461
Original Paper
Georg Thieme Verlag Stuttgart ·New York

Infection with Phloem Limited Abutilon Mosaic Virus Causes Localized Carbohydrate Accumulation in Leaves of Abutilon striatum: Relationships to Symptom Development and Effects on Chlorophyll Fluorescence Quenching During Photosynthetic Induction

G. Lohaus 1 , H. W. Heldt 1 , C. B. Osmond 2
  • 1 Biochemie der Pflanze, Albrecht-von-Haller-Institut für Pflanzenwissenschaften, Universität Göttingen, Göttingen, Germany
  • 2 Photobioenergetics Group, Research School of Biological Sciences, Institute of Advanced Studies, The Australian National University, Weston Creek, Australia
Further Information

Publication History

September 6, 1999

January 11, 2000

Publication Date:
31 December 2000 (online)

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Abstract:

Infection with phloem limited Abutilon Mosaic Virus caused localized carbohydrate accumulation (high levels of starch, sucrose, and hexoses) in leaves of Abutilon striatum during early symptom development. In mature leaves with attenuated symptoms, tissues showing faint vein-clearing had markedly higher carbohydrate contents than uniformly green areas of the same leaf. A similar pattern of carbohydrate accumulation was found in pale-green mosaics in mature leaves with overt symptoms when compared to green-islands of the same leaf, but overnight carbohydrate loses were comparable to controls. Because leaves with attenuated symptoms showed no further symptom development whereas the pale-green mosaics became yellow and eventually necrotic in leaves with overt symptoms, it seems unlikely that carbohydrate accumulation following impaired translocation was responsible for symptom expression. High carbohydrate status in leaves with attenuated symptoms had little effect on nonphotochemical quenching during early stages of photosynthetic induction. In leaves with overt symptoms, areas of high carbohydrate status with pale-green mosaics showed markedly slower nonphotochemical quenching. Early symptom areas of young leaves, and advanced symptom areas of mature leaves had low starch contents but were otherwise similar to controls in carbohydrate status. Impaired nonphotochemical quenching in these tissues tended to reflect the state of symptom development, rather than carbohydrate status. Plants with overt symptoms grew about half as fast as plants with attenuated symptoms.

Abbreviations:

AbMV: Abutilon Mosaic Virus Chl: chlorophyll FM: maximum Chl fluorescence in the first saturating pulse after dark-adaptation FMŒ: maximum Chl fluorescence in subsequent saturating pulses applied during transients NPQ: nonphotochemical quenching (FM/FMŒ − 1)

Key words:

Fluorescence imaging - hexose accumulation - nonphotochemical quenching - phloem transport - sugar accumulation

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C. B. Osmond

Photobioenergetics Group Research School of Biological Sciences Institute of Advanced Studies The Australian National University

Box 3252

Weston Creek ACT 2611

Australia

Section Editor: U. Lüttge

Email: osmond@rsbs.anu.edu.au

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