A Phosphopeptide Corresponding to the Cytosolic Stretch Connecting Transmembrane Segments 8 and 9 of the Plasma Membrane H⁺-ATPase Binds 14-3-3 Proteins and Inhibits Fusicoccin-Induced Activation of the H⁺-ATPase

Abstract:

A putative consensus domain for binding of 14-3-3 proteins to the plasma membrane (PM) H⁺-ATPase was identified in the highly-conserved sequence RSR(p)SWSF [where (p)S is Ser776 of the maize isoform MHA2], localized in the cytosolic stretch connecting transmembrane segments 8 and 9. A 15 amino acid biotinylated phosphopeptide comprising this motif: i) bound a recombinant 14-3-3 protein, ii) inhibited fusicoccin-induced stimulation of the PM H⁺-ATPase activity both in PM isolated from germinating radish (Raphanus sativus L.) seedlings and in ER isolated from Saccharomyces cerevisiae expressing AHA1 (an isoform of Arabidopsis thaliana PM H⁺-ATPase), and iii) inhibited fusicoccin binding to PM isolated from germinating radish seedlings. The corresponding non-phosphorylated peptide was inactive in all the performed assays. Together, these results suggest that the cytosolic strand connecting transmembrane segments 8 and 9 of the PM H⁺-ATPase is a 14-3-3 binding site which might cooperate with the C-terminal domain of the’enzyme in generating a stable association between the H⁺-ATPase and 14-3-3 protein.

Abbreviations:

Brij 58: polyoxyethylene 20 cetyl ether BTP: bis-tris propane (1,3-bis[tris(hydroxymethyl)methylamino]propane) FC: fusicoccin HEPES: 4-(2-hydroxymethyl)-1-piperazineethanesulfonic acid MES: 2-(N-morpholino)ethanesulfonic acid MOPS: 3-(N-morpholino)propanesulfonic acid PM: plasma membrane

Key words:

Plasma membrane H⁺-ATPase - 14-3-3 protein - fusicoccin

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