Suppression of Rat Neutrophil Reactive Oxygen Species Production and Adhesion by the Diterpenoid Lactone Andrographolide

Abstract

The present study was to examine whether andrographolide, a diterpenoid lactone isolated from the anti-inflammatory herbal medicine Andrographis paniculata (Burm. f.) Nees. (Acanthaceae), has the ability to prevent phorbol-12-myristate-13-acetate (PMA)-induced reactive oxygen species (ROS) production, as well as N-formyl-methionyl-leucyl-phenylalanine (fMLP)-induced adhesion by rat neutrophils. Results demonstrated that PMA (100 ng/ml) induced rapid accumulation of H₂O₂ and O₂ ^· in neutrophils within 30 minutes. Andrographolide (0.1 to 10 μM) pretreatment (10 min, 37 °C) significantly attenuated the accumulation of these two oxygen radical metabolites. Administration of andrographolide also significantly prevented fMLP-induced neutrophil adhesion. These data suggest that preventing ROS production and neutrophils adhesion may confer andrographolide the ability to be an anti-inflammatory drug.

Abbreviations

PMA:phorbol-12-myristate-13-acetatefMLP:N-formyl-methionyl-leucyl-phenylalanineROS:reactive oxygen species

Andrographis paniculata - Acanthaceae - andrographolide - neutrophil - phorbol-12-myristate-13-acetate - reactive oxygen species - N-formyl-methionyl-leucyl-phenylalanine - adhesion

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