Abstract
A morphologically distinct population (var. almeriensis) of the lichen species Diploschistes ocellatus from southern Spain is examined using morphological and molecular methods. While
the morphological examination revealed some unique features of var. almeriensis, the ITS sequence data did not support a distinction from D. ocellatus s. str. The Kishino-Hasegawa test did not rule out trees distinguishing between the
two morphotypes, although spectral analytical data support an inclusion of var. almeriensis in D. ocellatus. The influence of potentially erronously aligned ambiguous sites is evaluated by analysis
of different alignments. However, all alignments agree in placing all specimens of
D. ocellatus together and, consequently, in the consensus tree D. ocellatus s. lat. forms a monophyletic clade with high bootstrap support.
Key words
Lichens - Ascomycotina - Ostropales -
Diploschistes
- ribosomal DNA - molecular phylogeny - morphology - species
References
- 01
Berbee, M. L., and Taylor, J. W..
(1992);
Two Ascomycete classes based on fruiting-body characters and ribosomal DNA sequences.
Mol. Biol. Evol..
9
278-284
- 02
Bowman, B. H.,, Taylor, J. W.,, Brownlee, A. G.,, Lee, J.,, Lu, S. D.,, and White,
T. J..
(1992);
Molecular evolution of the fungi: relationship of the Basidiomycetes, Ascomycetes
and Chytridiomycetes.
Mol. Biol. Evol..
9
285-296
- 03 Charleston, M. A., and Page, D. M.. (1996) Spectrum 2.0 User's Guide. Glasgow;
Glasgow Univ. pp. 1-7
- 04
Farris, J. S.,, Källersjö, M.,, Crowe, T.,, Lipscomb, D.,, and Johansson, U..
(1999);
Frigatebirds, tropicbirds and Ciconiida: excesses and of confidence probability.
Cladistics.
15
1-7
- 05
Felsenstein, J..
(1981);
Evolutionary trees from DNA sequences: a maximum likelihood approach.
J. Mol. Evol..
17
368-376
- 06
Felsenstein, J..
(1985);
Confidence limits on phylogenies: an approach using the bootstrap.
Evolution.
39
783-791
- 07
Friedlander, T. P.,, Regier, J. C.,, and Mitter, C..
(1994);
Phylogenetic information content of five nuclear gene sequences in animals: initial
assessment of character sets from concordance and divergence studies.
Syst. Biol..
43
511-525
- 08
Gardes, M., and Bruns, T. D..
(1993);
ITS primers with enhanced specifity for basidiomycetes - application to the identification
of mycorrhizae and rusts.
Mol. Ecol..
2
113-118
- 09
Gargas, A., and DePriest, P. T..
(1996);
A nomenclature for fungal PCR primers with examples from intron-containing SSU rDNA.
Mycologia.
88
745-748
- 10
Gatesy, J.,, DeSalle, R.,, and Wheeler, W..
(1993);
Alignment-ambiguous nucleotide sites and the exclusion of systematic data.
Mol. Phylogen. Evol..
2
152-157
- 11
Hendy, M. D., and Penny, D..
(1993);
Spectral analysis of phylogenetic data.
J. Class..
10
5-24
- 12
Huelsenbeck, J. P., and Crandall, K. A..
(1997);
Phylogeny estimation and hypothesis testing using maximum likelihood.
Ann. Rev. Ecol. and Syst..
28
437-466
- 13
Huelsenbeck, J. P., and Rannala, B..
(1997);
Phylogenetic methods come of age: testing hypotheses in an evolutionary context.
Science.
276
227-232
- 14
Kishino, H., and Hasegawa, M..
(1989);
Evaluation of the maximum likelihood estimate of the evolutionary tree topologies
from DNA sequence data, and the branching order in Hominoidea.
J. Mol. Evol..
29
170-179
- 15
Lento, G. M.,, Hickson, R E.,, Chambers, G. K.,, and Penny, D..
(1995);
Use of spectral analysis to test hypotheses on the origin of pinnipeds.
Mol. Biol. Evol..
12
28-52
- 16
Llimona, X..
(1974);
Las comunidades de liquenes de los yesos de España.
Opusc. sparsa.
6
1-18
- 17
Lohtander, K.,, Källersjö, M.,, and Tehler, A..
(1998 a);
Dispersal strategies in Roccellina capensis (Arthoniales).
Lichenologist.
30
341-350
- 18
Lohtander, K.,, Myllys, L.,, Sundin, R.,, Källersjö, M.,, and Tehler, A..
(1998 b);
The species pair concept in the lichen Dendrographa leucophaea (Arthoniales): analyses based on ITS sequences.
Bryologist.
101
404-411
- 19
Lockhart, P. J.,, Steel, M. A.,, Hendy, M. D.,, and Penny, D..
(1994);
Recovering evolutionary trees under a more realistic model of sequence evolution.
Mol. Biol. Evol..
11
605-612
- 20
Lumbsch, H. T..
(1989);
Die holarktischen Vertreter der Flechtengattung Diploschistes (Thelotremataceae).
Journ. Hattori Bot. Lab..
66
133-196
- 21
Lumbsch, H. T., and Tehler, A..
(1998);
A cladistic analysis of the genus Diploschistes (lichenized Ascomycotina; Thelotremataceae).
Bryologist.
101
398-403
- 22
Martín, M. P., and Winka, W..
(2000);
Alternative methods for extracting and amplifying DNA from lichens.
Lichenologist.
32
189-196
- 23
Mattsson, J. E., and Lumbsch, H. T..
(1989);
The use of the species pair concept in lichen taxonomy.
Taxon.
38
238-241
- 24
Mindell, D. P., and Honeycutt, R. L..
(1990);
Ribosomal RNA in vertebrates: evolution and phylogenetic applications.
Ann. Rev. Ecol. Syst..
21
541-566
- 25
Myllys, L.,, Lohtander, K.,, Källerjö, M.,, and Tehler, A..
(1999 a);
Sequence insertions and ITS data provide congruent information on Roccella canariensis and R. tuberculata (Arthoniales, Euascomycetes) phylogeny.
Mol. Phylogen. Evol..
12
295-309
- 26
Myllys, L.,, Lohtander, K.,, Källerjö, M.,, and Tehler, A..
(1999 b);
Applicability of ITS data in Roccellaceae (Arthoniales, Euascomycetes) phylogeny.
Lichenologist.
31
461-476
- 27
Page, R. D. M..
(1996);
Treeview: An application to display phylogenetic trees on personal computers.
Computer Appl. Biosci..
12
357-358
- 28
Penny, D.,, Hasegawa, M.,, Waddell, P. J.,, and Hendy, M. D..
(1999);
Mammalian evolution: timing and implications from using the LogDeterminant transform
for proteins of differing amino acid composition.
Syst. Biol..
48
76-93
- 29
Poelt, J..
(1970);
Das Konzept der Artenpaare bei den Flechten.
Vortr. Gesamtg. Bot., N.F. [Deutsch. Bot. Ges.].
4
187-198
- 30
Poelt, J..
(1972);
Die taxonomische Behandlung von Artenpaaren bei den Flechten.
Bot. Not..
125
77-81
- 31
Posada, D., and Crandall, K. A..
(1998);
MODELTEST: testing the model of DNA substitution.
Bioinformatics.
9
817-818
- 32
Steiner, G., and Müller, M..
(1996);
What can 18S rDNA do for bivalve phylogeny?.
J. Mol. Evol..
43
58-70
- 33
Strimmer, K., and Haeseler, A. von.
(1996);
Quartet-puzzling: a quartet maximum likelihood method for reconstructing tree topologies.
Mol. Biol. Evol..
13
964-969
- 34 Strimmer, K., and Haeseler, A. von. (1997) Puzzle. Maximum likelihood analysis
for nucleotide, amino acid, and two-state data. Version 4.0. München; Univ. München
p. 16
- 35 Swofford, D. L.. (1998) PAUP*. Phylogenetic analysis using parsimony (*and other
methods). Version 4. Sunderland, Mass.; Sinauer Associates p. 128
- 36
Tamura, K., and Nei, M..
(1993);
Estimation of the number of nucleotide substitutions in the control region of mitochondrial
DNA in humans and chimpanzees.
Mol. Biol. Evol..
10
512-526
- 37
Tehler, A..
(1982);
The species pair concept in lichenology.
Taxon.
31
708-717
- 38
Thompson, J. D.,, Higgins, D. G.,, and Gibson, T. J..
(1994);
Clustal W: improving the sensitivity of progressive multiple sequence alignment through
sequence weighting, position-specific gap penalties and weight matrix choice.
Nucl. Acids Res..
22
4673-4680
- 39
Wenzel, J. W., and Siddall, M. E..
(1999);
Noise.
Cladistics.
15
51-67
- 40
Wheeler, W. C..
(1995);
Sequence alignment, parameter sensitivity, and the phylogenetic analysis of molecular
data.
Syst. Biol..
44
321-331
- 41 White, T. J.,, Bruns, T.,, Lee, S.,, and Taylor, J.. (1990)
Amplification and direct sequencing of fungal ribosomal RNA genes for phylogenetics. PCR Protocols: a Guide to Methods and Applications. Innis, M. A., Gelfand, D. H.,
Sninsky, J. J. and White, T. J., eds. New York; Academic Press p. 315-322
- 42
Winka, K.,, Ahlberg, C.,, and Eriksson, O. E..
(1998);
Are there lichenized Ostropales?.
Lichenologist.
30
455-462
- 43
Yang, Z..
(1994);
Maximum likelihood phylogenetic estimation from DNA sequences with variable rates
over sites: approximate methods.
J. Mol. Evol..
39
306-314
H. T. Lumbsch
Botanisches Institut Universität Essen
45117 Essen Germany
eMail: lumbsch@uni-essen.de
Section Editor: H. M. Jahns