Endoscopic treatments with Conditioned medium from adipose-derived mesenchymal stem cells for non-steroidal anti-inflammatory drugs (NSAID)-related gastric ulcer

Aims This study aims to assess conditioned medium's potential impact from adipose-derived mesenchymal stem cells (MSCs) on NSAID-induced gastric ulcers in a pig model.

Methods Endoscopic injection of conditioned medium derived from adipose mesenchymal stem cells in a porcine model of NSAID-induced gastric ulcers. This model is established through the continue administration of indomethacin and the creation of artificial gastric ulcers using endoscopic submucosal dissection surgery under general anesthesia [1].

Results Endoscopic surveillance indicated that the injection of hypo-conditioned medium (Hypo-CM) significantly enhanced the healing of NSAID-related gastric ulcers compared to the control group at both days 3 and 7. On day 3, the ulcer index in the Hypo-CM treated group was reduced by 54.33%, compared to 115.2% in the control group (P=0.0027). By day 7, the ulcer index for the Hypo-CM group further decreased to 11.04%, significantly lower than the control group’s 59.19% (P<0.05). Additionally, at day 7, the Hypo-CM treatment promoted greater ulcer healing than the Omeprazole group, which had an ulcer index of 42.03% (P<0.05).

Conclusions Endoscopic injection of hypo-conditioned medium from adipose mesenchymal stem cells exhibits therapeutic effects on NSAID-associated gastric ulcers by stimulating cell proliferation, enhancing angiogenesis, and promoting collagen production and transformation.

Conflicts of Interest

Authors do not have any conflict of interest to disclose.

• References

• 1 Xia X, Chan KF, Wong GTY, Wang P, Liu L, Yeung BPM, Ng EKW, Lau JYW, Chiu PWY.. Mesenchymal stem cells promote healing of nonsteroidal anti-inflammatory drug-related peptic ulcer through paracrine actions in pigs. Sci Transl Med 2019; 11 (516): eaat7455

Publication History

Article published online:
27 March 2025