Planta Med 2023; 89(14): 1370
DOI: 10.1055/s-0043-1774101
Abstracts
Tuesday 4th July 2023 | Poster Session II
Phytochemistry II - Antimicrobials; Cosmetics; Essential oils; Nutraceuticals; Biotechnology

Enhancement of bergenin formation in cell suspension cultures of Bergenia pacumbis (Buch.-Ham. ex D.Don) C.Y.Wu & J.T.Pan by bacterial endophytes

Christoph Wawrosch
1   University of Vienna, Dept. of Pharmaceutical Sciences, Division of Pharmacognosy, Vienna, Austria
,
Stefan Steinbrecher
1   University of Vienna, Dept. of Pharmaceutical Sciences, Division of Pharmacognosy, Vienna, Austria
,
Martina Oberhofer
1   University of Vienna, Dept. of Pharmaceutical Sciences, Division of Pharmacognosy, Vienna, Austria
,
Sergey Zotchev
1   University of Vienna, Dept. of Pharmaceutical Sciences, Division of Pharmacognosy, Vienna, Austria
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Various species of the genus Bergenia (Saxifragaceae) are used as an Ayurvedic traditional remedy for the treatment of e.g. heart, lung, kidney, liver and bladder diseases, as well as tumours and ulcers [1]. The gallic acid derivative bergenin, a main compound of Bergenia species, exhibits many biological activities like hepatoprotective, anti-inflammatory, antioxidant, antitumour, or anti-virus [2]. Endophytes, microorganisms that live within plants without causing any visible disease symptoms, can, amongst other benefits for the host plant, influence the production of plant secondary metabolites [3]. In this study, cell suspension cultures of Bergenia pacumbis were established in order to investigate the effects of bacterial endophytes on bergenin formation in vitro. Cultures were treated with cells and culture filtrates of 5 bacterial strains (Microbacterium sp., Micrococcus sp., Moraxella sp., Paracoccus sp. and Pseudomonas sp.), which were earlier isolated from B. pacumbis and grown in two different media. Elicitation of Bergenia cell suspension cultures with cells of Moraxella sp. cultivated in LB medium, or with the culture filtrate of Micrococcus sp. cultivated in TSB medium, was most effective and resulted in ca. 2.7 fold increase in bergenin formation compared to the untreated control. Our results indicate that elicitation of plant suspension cultures with specific bacterial endophytes might be a promising strategy to increase in vitro secondary metabolite production.



Publication History

Article published online:
16 November 2023

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