Variations of Redox Balance in Different Stages of Childhood Immune Thrombocytopenic Purpura

Rasa H. Medovic; Ivan M. Srejovic; Marija V. Medovic; Isidora M. Milosavljevic; Marina R. Nikolic; Aleksandra Z. Stojanovic; Milos B. Kuzmanovic; Predrag M. Djurdjevic; Sergey B. Bolevich; Vladimir P. Fisenko; Vladimir Lj. Jakovljevic; Zoran R. Igrutinovic

doi:10.1055/s-0043-1772683

Thrombosis and Haemostasis, Inhaltsverzeichnis

Thromb Haemost 2023; 123(12): 1129-1139
DOI: 10.1055/s-0043-1772683

Cellular Haemostasis and Platelets

Variations of Redox Balance in Different Stages of Childhood Immune Thrombocytopenic Purpura

Rasa H. Medovic

¹Department of Pediatrics, Faculty of Medical Sciences, University of Kragujevac, Kragujevac, Serbia

²University Clinical Center Kragujevac, Kragujevac, Serbia

,

Ivan M. Srejovic

³Department of Physiology, Faculty of Medical Sciences, University of Kragujevac, Kragujevac, Serbia

⁴Center of Excellence for the Study of Redox Balance in Cardiovascular and Metabolic Disorders, University of Kragujevac, Kragujevac, Serbia

⁵Department of Pharmacology, First Moscow State Medical University I.M. Sechenov, Moscow, Russian Federation

,

Marija V. Medovic

²University Clinical Center Kragujevac, Kragujevac, Serbia

⁶Department of Dermatovenerology, Faculty of Medical Sciences, University of Kragujevac, Kragujevac, Serbia

,

Isidora M. Milosavljevic

⁴Center of Excellence for the Study of Redox Balance in Cardiovascular and Metabolic Disorders, University of Kragujevac, Kragujevac, Serbia

⁷Department of Pharmacy, Faculty of Medical Sciences, University of Kragujevac, Kragujevac, Serbia

,

Marina R. Nikolic

³Department of Physiology, Faculty of Medical Sciences, University of Kragujevac, Kragujevac, Serbia

⁴Center of Excellence for the Study of Redox Balance in Cardiovascular and Metabolic Disorders, University of Kragujevac, Kragujevac, Serbia

,

Aleksandra Z. Stojanovic

⁴Center of Excellence for the Study of Redox Balance in Cardiovascular and Metabolic Disorders, University of Kragujevac, Kragujevac, Serbia

⁷Department of Pharmacy, Faculty of Medical Sciences, University of Kragujevac, Kragujevac, Serbia

,

Milos B. Kuzmanovic

⁸Mother and Child Health Institute of Serbia “Dr Vukan Cupic”, Belgrade, Serbia

⁹Department of Pediatrics, Medical Faculty, University of Belgrade, Belgrade, Serbia

,

Predrag M. Djurdjevic

²University Clinical Center Kragujevac, Kragujevac, Serbia

¹⁰Department of Internal medicine, Faculty of Medical Sciences, University of Kragujevac, Kragujevac, Serbia

,

Sergey B. Bolevich

¹¹Department of Human Pathology, First Moscow State Medical University I.M. Sechenov, Moscow, Russia Federation

,

Vladimir P. Fisenko

⁵Department of Pharmacology, First Moscow State Medical University I.M. Sechenov, Moscow, Russian Federation

,

Vladimir Lj. Jakovljevic

³Department of Physiology, Faculty of Medical Sciences, University of Kragujevac, Kragujevac, Serbia

⁴Center of Excellence for the Study of Redox Balance in Cardiovascular and Metabolic Disorders, University of Kragujevac, Kragujevac, Serbia

¹¹Department of Human Pathology, First Moscow State Medical University I.M. Sechenov, Moscow, Russia Federation

,

Zoran R. Igrutinovic

¹Department of Pediatrics, Faculty of Medical Sciences, University of Kragujevac, Kragujevac, Serbia

²University Clinical Center Kragujevac, Kragujevac, Serbia

› Institutsangaben

Abstract

Background Few previous studies indicated the role of oxidative stress in the pathogenesis of childhood idiopathic thrombocytopenic purpura (ITP), but there are little data regarding changes in redox balance in different forms of the disease, and changes after therapeutic procedures. We aimed to investigate the values of pro-oxidants and antioxidative capacity in various forms of ITP before and after the applying therapy.

Materials and Methods The research included 102 children, classified into the following groups: (1) newly diagnosed ITP (ndITP), (2) persistent ITP, (3) chronic ITP (chITP), and (4) control groups: (A) healthy control and (B) previously experienced ITP—healthy children who had been suffering from ITP earlier. During the clinical assessment, a blood sample was taken from the patients, from which the value of pro-oxidants (index of lipid peroxidation measured as TBARS, nitrites [NO₂ ⁻], as measurement of nitric oxide [NO] production, superoxide anion radical [O₂ ⁻], and hydrogen peroxide [H₂O₂]) and the capacity of antioxidant protection (activity of superoxide dismutase and catalase, and quantity of reduced glutathione) were determined spectrophotometrically.

Results Our results demonstrated that values of pro-oxidants, especially reflected through the TBARS and O₂ ⁻, were the highest in the ndITP and exacerbated chITP groups. Also, the activity of the endogenous antioxidative defense system was the lowest in these groups. Intravenous immunoglobulin therapy in the ndITP group exerted the most prominent effect on the redox balance.

Conclusion It can be concluded that severity and exacerbation of the ITP are closely related to the redox status.

Keywords

childhood idiopathic thrombocytopenic purpura - corticosteroids - endogenous antioxidant - intravenous immunoglobulins - oxidative stress

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Referenzen

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