Tierarztl Prax Ausg G Grosstiere Nutztiere 2023; 51(03): 1
DOI: 10.1055/s-0043-1770826
Abstracts | DVG
Vorträge

CK8+ alveolar intermediate differentiation cells are features of alveolar regeneration in SARS-CoV-2 infected hamsters

F Armando
1   Department of Pathology, University of Veterinary Medicine, Foundation, Hannover, Germany
,
L Heydemann
1   Department of Pathology, University of Veterinary Medicine, Foundation, Hannover, Germany
,
G Beythien
1   Department of Pathology, University of Veterinary Medicine, Foundation, Hannover, Germany
,
K Becker
1   Department of Pathology, University of Veterinary Medicine, Foundation, Hannover, Germany
,
K Schughart
2   Immunology and Biochemistry, University of Tennessee, Health Science Center, Memphis, Tennessee, USA
3   Institute of Virology Münster, University of Münster, Münster, Germany
,
S Stanelle-Bertram
4   Department for Viral Zoonoses-One Health, Leibniz Institute for Virology, Hamburg, Germany
,
B Schaumburg
4   Department for Viral Zoonoses-One Health, Leibniz Institute for Virology, Hamburg, Germany
,
N Mounogou-Kouassi
4   Department for Viral Zoonoses-One Health, Leibniz Institute for Virology, Hamburg, Germany
,
S Beck
4   Department for Viral Zoonoses-One Health, Leibniz Institute for Virology, Hamburg, Germany
,
M Zickler
4   Department for Viral Zoonoses-One Health, Leibniz Institute for Virology, Hamburg, Germany
,
M Kühnel
5   Institute of Pathology, Hannover Medical School (MHH), Hannover, Germany
,
G Gabriel
4   Department for Viral Zoonoses-One Health, Leibniz Institute for Virology, Hamburg, Germany
,
A Beineke
1   Department of Pathology, University of Veterinary Medicine, Foundation, Hannover, Germany
,
W Baumgärtner
1   Department of Pathology, University of Veterinary Medicine, Foundation, Hannover, Germany
,
M Ciurkiewicz
1   Department of Pathology, University of Veterinary Medicine, Foundation, Hannover, Germany
› Author Affiliations
 
 

    Einleitung A relevant number of coronavirus disease 2019 (COVID-19) survivors suffers from post-acute sequelae of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) (PASC). Current evidence suggests a dysregulated alveolar regeneration in COVID-19 as a possible explanation for respiratory PASC symptoms.

    Material und Methoden This study investigates morphologic and transcriptomic features of alveolar regeneration in SARS-CoV-2 infected Syrian golden hamsters.

    Befunde We demonstrate that CK8+ alveolar differentiation intermediate (ADI) cells accumulate following SARS-CoV-2-induced diffuse alveolar damage. A subset of ADI cells shows nuclear accumulation of p53 at 6- and 14-days post infection (dpi), indicating a prolonged block in the ADI state. Transcriptome data shows the expression of gene signatures driving ADI cell senescence, epithelial-mesenchymal transition, and angiogenesis. At 14 dpi, persistence of ADI cells, M2-type macrophages, and subpleural fibrosis is observed, indicating incomplete alveolar restoration.

    Schlussfolgerungen The results demonstrate that the hamster model reliably phenocopies indicators of a dysregulated alveolar regeneration of COVID-19 patients.


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    Publication History

    Article published online:
    11 August 2023

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