Z Gastroenterol 2023; 61(05): e192-e193
DOI: 10.1055/s-0043-1769043
Abstracts | ÖGGH
POSTER
Gastroenterologie

Establishment of an in vitro microbiome model of the human gut microbiome using the DASbox mini bioreactor system

K. Žukauskaitė
1   Department of Gastroenterology and Hepatology, Medical University of Graz, Graz, Austria
2   Institute of Biosciences, Life Science Center, Vilnius University, Vilnius, Lithuania
,
C. Pacher
1   Department of Gastroenterology and Hepatology, Medical University of Graz, Graz, Austria
3   Center for Biomarker Research in Medicine, Graz, Austria
,
S. Kofler
3   Center for Biomarker Research in Medicine, Graz, Austria
4   Division of Medical Psychology, Psychosomatics and Psychotherapeutic Medicine, Medical University of Graz, Graz, Austria.
,
I. Balazs
1   Department of Gastroenterology and Hepatology, Medical University of Graz, Graz, Austria
3   Center for Biomarker Research in Medicine, Graz, Austria
,
A. Horvath
1   Department of Gastroenterology and Hepatology, Medical University of Graz, Graz, Austria
3   Center for Biomarker Research in Medicine, Graz, Austria
,
V. Stadlbauer
1   Department of Gastroenterology and Hepatology, Medical University of Graz, Graz, Austria
3   Center for Biomarker Research in Medicine, Graz, Austria
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Background  The human gut microbiome has an immense part in human health. Disruption of the gut microbiome (or dysbiosis) is associated with various intestinal conditions, including obesity, inflammatory bowel disease, and even cancer. Understanding the strong demand for a platform to study human gut microbiome, we focus on the establishment of a novel in vitro model for the human gut that could be implemented into clinical practice.

Aim  The main goal of this study was to optimize sampling and culture conditions, including the possible time between sample collection and culture and the choice of the medium, to resemble physiological conditions in the gut.

Methods  The stool collection procedure was performed using an anaerobic microbiome collection kit GutAlive (MicroViable therapeutics, Spain). The stool samples were stored in four GutAlive containers for less than 1 hour, 2, 24, and 48 hours after sampling. Stool samples were prepared anaerobically and then cultured in a DASbox mini bioreactor system (Eppendorf, Germany) for 5 days. Bryant and Burkey (BB), or continuous flow fermentation (CFF) medium were tested as potential medium composition. Every 24 hours, cell viability was evaluated, and samples were analyzed with a flow cytometry-based microbial fingerprinting approach. pH, temperature, and dissolved oxygen were measured continuously within the bioreactors.

Results  Flow cytometry analysis revealed that sample storage in anaerobic microbiome collection containers did not have strong influence on microbial community structure for up to 48 hours. According to the results, the BB medium caused a higher pH than the CFF medium. It resulted in significantly higher cell viability and more stable pH values.

Conclusion  In conclusion, samples from patients can be stored for up to two days in GutAlive container without significant changes in the microbiome structure. BB medium is more suitable to maintain the microbial structure and physiological pH of the distal colon.



Publication History

Article published online:
24 May 2023

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