Klin Padiatr 2023; 235(03): 197
DOI: 10.1055/s-0043-1768524
Abstracts

Spatial analysis reveals distinct immune phenotypes and tertiary lymphoid structure-like aggregates in pediatric AML

J B Koedijk
1   Princess Máxima Center for Pediatric Oncology, 3584 CS Utrecht, The Netherlands
2   Department of Pediatric Oncology, Erasmus MC/Sophia Children's Hospital, 3015 GD Rotterdam, The Netherlands
,
I van der Werf
1   Princess Máxima Center for Pediatric Oncology, 3584 CS Utrecht, The Netherlands
3   Oncode Institute, 3521 AL, Utrecht, The Netherlands
,
M A Vermeulen
1   Princess Máxima Center for Pediatric Oncology, 3584 CS Utrecht, The Netherlands
,
A Perzolli
1   Princess Máxima Center for Pediatric Oncology, 3584 CS Utrecht, The Netherlands
2   Department of Pediatric Oncology, Erasmus MC/Sophia Children's Hospital, 3015 GD Rotterdam, The Netherlands
,
M Fiocco
1   Princess Máxima Center for Pediatric Oncology, 3584 CS Utrecht, The Netherlands
4   Mathematical Institute, Leiden University, Leiden, The Netherlands
5   Department of Biomedical Data Sciences, Leiden University Medical Center, Leiden, The Netherlands
,
H A de Groot-Kruseman
1   Princess Máxima Center for Pediatric Oncology, 3584 CS Utrecht, The Netherlands
,
S Nierkens
1   Princess Máxima Center for Pediatric Oncology, 3584 CS Utrecht, The Netherlands
6   Center for Translational Immunology, University Medical Center Utrecht, 3584 CX Utrecht, The Netherlands
,
M E Belderbos
1   Princess Máxima Center for Pediatric Oncology, 3584 CS Utrecht, The Netherlands
,
C M Zwaan
1   Princess Máxima Center for Pediatric Oncology, 3584 CS Utrecht, The Netherlands
2   Department of Pediatric Oncology, Erasmus MC/Sophia Children's Hospital, 3015 GD Rotterdam, The Netherlands
,
O Heidenreich
1   Princess Máxima Center for Pediatric Oncology, 3584 CS Utrecht, The Netherlands
7   Wolfson Childhood Cancer Research Centre, Newcastle University, Newcastle upon Tyne NE1 7RU, UK.
› Institutsangaben
 
 

    Pediatric cancers are characterized by a relatively low mutational burden and therefore, children are thought to be poor candidates for T cell-engaging immunotherapies. Here, we performed a multidimensional characterization of the tumor immune microenvironment in newly diagnosed children with acute myeloid leukemia (AML) and non-leukemic controls. We identified a subset of pediatric AML patients with remarkably high levels of T cell infiltration and a relatively low abundance of anti-inflammatory macrophages in the bone marrow. In addition, we detected large T cell networks that colocalized with B cells in immune-infiltrated samples, resembling tertiary lymphoid structures as described in solid tumors. Using spatial transcriptomics, we dissected the composition of these structures and revealed unique hotspots of anti-tumor immunity. This work raises the possibility that a subset of pediatric AML patients may benefit from T cell-engaging immunotherapies and encourages further study of these lymphoid structures in the context of immunotherapy in AML.


    Publikationsverlauf

    Artikel online veröffentlicht:
    12. Mai 2023

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