Systemic Hypoxia Increases the Expression of DPP4 in Preadipocytes of Healthy Human Participants

Helena H. Chowdhury; Jelena Velebit; Igor B. Mekjavic; Ola Eiken; Marko Kreft; Robert Zorec

doi:10.1055/s-0043-113451

Experimental and Clinical Endocrinology & Diabetes, Inhaltsverzeichnis

Exp Clin Endocrinol Diabetes 2018; 126(02): 91-95
DOI: 10.1055/s-0043-113451

Article

Systemic Hypoxia Increases the Expression of DPP4 in Preadipocytes of Healthy Human Participants

Helena H. Chowdhury

¹Laboratory for Neuroendocrinology - Molecular Cell Physiology, Institute of Pathophysiology, Faculty of Medicine, University of Ljubljana, Zaloška 4, Ljubljana, Slovenia

²Celica Biomedical, Tehnološki park 24, Ljubljana, Slovenia

,

Jelena Velebit

¹Laboratory for Neuroendocrinology - Molecular Cell Physiology, Institute of Pathophysiology, Faculty of Medicine, University of Ljubljana, Zaloška 4, Ljubljana, Slovenia

²Celica Biomedical, Tehnološki park 24, Ljubljana, Slovenia

,

Igor B. Mekjavic

³Department of Automation, Biocybernetics and Robotics, Jožef Stefan Institute, Jamova 39, Ljubljana, Slovenia

,

Ola Eiken

⁴Department of Environmental Physiology, Swedish Aerospace Physiology Centre, Royal Institute of Technology, Stockholm, Sweden

,

Marko Kreft

¹Laboratory for Neuroendocrinology - Molecular Cell Physiology, Institute of Pathophysiology, Faculty of Medicine, University of Ljubljana, Zaloška 4, Ljubljana, Slovenia

²Celica Biomedical, Tehnološki park 24, Ljubljana, Slovenia

⁵Department of Biology, Biotechnical Faculty, University of Ljubljana, Večna pot 111, Ljubljana, Slovenia

,

Robert Zorec

¹Laboratory for Neuroendocrinology - Molecular Cell Physiology, Institute of Pathophysiology, Faculty of Medicine, University of Ljubljana, Zaloška 4, Ljubljana, Slovenia

²Celica Biomedical, Tehnološki park 24, Ljubljana, Slovenia

› Institutsangaben

Abstract

Dipeptidyl peptidase 4 (DPP4) is a transmembrane glycoprotein involved in protein degradation. Due to its action on incretins, which increase insulin secretion, DPP4 is considered a therapeutic target for type 2 diabetes. Here we have studied the role of single and combined effects of hypoxia and inactivity on the expression of DPP4 in human adipose tissue of 12 adult normal-weight males. Fat biopsies were obtained at baseline and after each of three experimental campaigns. The results revealed that in isolated human preadipocytes the expression of DPP4 was significantly increased by exposure of participants to hypoxia. Physical inactivity per se had no apparent effect on the DPP4 expression. It is concluded that DPP4 may be a marker to monitor indirectly tissue hypoxia, as occurs in obese subjects.

Key words

dipeptidyl peptidase 4 - adipose differentiation - hypoxia - stemness

Volltext

Referenzen

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