Transcriptional Coactivator p300 and Silent Information Regulator 1 (SIRT1) Gene Polymorphism Associated with Diabetic Kidney Disease in a Chinese Cohort

Abstract

Objective

One of the most serious complications of diabetes mellitus, which could lead to end-stage renal disease across all demographics, is ‘diabetic kidney disease’. However, how diabetic kidney disease develops remains unclear. Studies conducted thus far suggest that a major factor in the origination and development of the disease occurs through histone acetylation modifications. This study aims to examine the probable relationship in Chinese patients suffering from type 2 diabetes.

Method

A case–control study was conducted in the Chongqing region of China on the Chinese Han population. Patients suffering from type 2 diabetes mellitus were selected between March 2014 and Dec 2014 from the Department of Endocrinology, which is the First Affiliated Hospital of Chongqing Medical University. TaqMan probes were employed to perform an allelic discrimination assay for genotyping p300 and the SIRT1 (Silent Information Regulator 1) polymorphism. The risk factors diabetic kidney disease were determined by statistical analysis.

Results

The dispersion of the p300 genotype frequencies and SIRT1 gene polymorphism adheres to the Hardy-Weinberg equilibrium. The DKD group had a greater allele G frequency distribution, and allele G patients have a higher probability of diabetic kidney disease. Female patients, patients younger than 65 years of age, and those with the AG or GG genotype are more likely to develop diabetic kidney disease than patients with the AA phenotype. Patients with the AG or GG genotype are more likely to suffer from a severe diabetic kidney disease than patients with the AA genotype, particularly if the patients are older than 65 years of age. The SIRT1 rs4746720 allele C is a risk factor for urinary Alb/Cr. Allele G and the TC genotype patients are more likely to develop diabetic kidney disease, while allele G and TT genotype patients are more likely develop a severe diabetic kidney disease.

Conclusion

Transcriptional coactivator p300 gene polymorphism correlates with the development and advancement of diabetic kidney disease. Additionally, the SIRT1 gene collaborates with the p300 gene and participates in promoting albuminuria in type 2 diabetes mellitus patients.

Key words

Single nucleotide polymorphism - p300 gene - SIRT1 gene - diabetic kidney disease

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