Exp Clin Endocrinol Diabetes 2017; 125(03): 163-170
DOI: 10.1055/s-0042-119525
Article
© Georg Thieme Verlag KG Stuttgart · New York

Brown and White Adipose Tissue Expression of IL6, UCP1 and SIRT1 are Associated with Alterations in Clinical, Metabolic and Anthropometric Parameters in Obese Humans

Antônio Sérgio Barcala Jorge
1   Nucleus of Epidemiological and Molecular Research Catrumano, Health Research Laboratory, Health Science Post-graduate Programme. Universidade Estadual de Montes Claros, Montes Claros, MG, Brazil
,
Gislaine Candida Batista Jorge
1   Nucleus of Epidemiological and Molecular Research Catrumano, Health Research Laboratory, Health Science Post-graduate Programme. Universidade Estadual de Montes Claros, Montes Claros, MG, Brazil
,
Alanna Fernandes Paraíso
1   Nucleus of Epidemiological and Molecular Research Catrumano, Health Research Laboratory, Health Science Post-graduate Programme. Universidade Estadual de Montes Claros, Montes Claros, MG, Brazil
,
Raíssa M. Porto Franco
2   Medical School. Universidade Estadual de Montes Claros, Montes Claros, MG, Brazil
,
Lara Jhullian Tolentino Vieira
2   Medical School. Universidade Estadual de Montes Claros, Montes Claros, MG, Brazil
,
Aline Mourão Hilzenderger
4   Department of Pharmacodynamics, University of Florida, Gainesville, FL, USA
,
André Luiz Sena Guimarães
1   Nucleus of Epidemiological and Molecular Research Catrumano, Health Research Laboratory, Health Science Post-graduate Programme. Universidade Estadual de Montes Claros, Montes Claros, MG, Brazil
,
João Marcus Oliveira Andrade
1   Nucleus of Epidemiological and Molecular Research Catrumano, Health Research Laboratory, Health Science Post-graduate Programme. Universidade Estadual de Montes Claros, Montes Claros, MG, Brazil
,
Alfredo M. Batista De-Paula
1   Nucleus of Epidemiological and Molecular Research Catrumano, Health Research Laboratory, Health Science Post-graduate Programme. Universidade Estadual de Montes Claros, Montes Claros, MG, Brazil
,
Sergio Henrique S. Santos
1   Nucleus of Epidemiological and Molecular Research Catrumano, Health Research Laboratory, Health Science Post-graduate Programme. Universidade Estadual de Montes Claros, Montes Claros, MG, Brazil
3   Institute of Agricultural Sciences. Food Engineering College, Universidade Federal de Minas Gerais (UFMG), Minas Gerais, Brazil; Montes Claros, Minas Gerais, Brazil
› Author Affiliations
Further Information

Publication History

received 28 March 2016
first decision 01 June 2016

accepted 18 October 2016

Publication Date:
10 January 2017 (online)

Abstract

Aim: The present study aimed to analyze the expression of IL6, UCP1 and SIRT1 in adipose tissue (WAT and BAT) in association to clinical, metabolic and anthropometric parameters in obese humans.

Methods: WAT and BAT samples from obese patients (n=27) were collected. IL6, UCP1 and SIRT1 markers were measured by qRT-PCR. The association between IL6, UCP1 and SIRT1 mRNA expression and anthropometric and clinical parameters were evaluated, using appropriate statistical tests.

Results: Our results demonstrated that high levels of IL6 are associated with altered glucose levels in the WAT (p=0.01). In contrast, high levels of IL6 in the BAT were associated with decreased % fat (p=0.01) and fat weight (p=0.02) and increased mVO2 (p=0.02) and VO2 (p=0.02). For UCP1, a higher expression in the BAT was observed when compared to the WAT (p=0.0001). This gene expression was associated with lower values of BMI (p=0.03), % fat (P=0.02) and fat weight (P=0.02) and increased mVO2 (p=0.041) and VO2 (p=0.001). In the WAT, decreased levels of SIRT1 were associated with increased fat weight (p=0.02); in the BAT, associations were found for % fat (p=0.018) and mVO2 (p=0.03).

Conclusion: These results reveal different characteristics in the biological actions between WAT and BAT in obese humans. Increased levels of IL6, UCP1 and SIRT1 in the BAT were associated with metabolic parameters improvements.

 
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