No Evidence of Racial Differences in Endothelial Function and Exercise Blood Flow in Young, Healthy Males Following Acute Antioxidant Supplementation

Abstract

This study investigated the effects of acute antioxidant supplementation on endothelial function, exercise blood flow and oxidative stress biomarkers in 9 young African American compared to 10 Caucasian males (25.7±1.2 years). We hypothesized that African American males would have lower exercise blood flow and endothelial responsiveness compared to Caucasian males, and these responses would be improved following antioxidant supplementation. Ultrasonography was used to measure blood flow during handgrip exercise. Endothelial function was assessed using flow-mediated dilation, and lipid peroxidation was assessed by measuring levels of malondialdehyde-thiobarbituric acid reactive substances. African American males exhibited lower endothelial function than Caucasians at baseline (8.3±1.7 vs. 12.2±1.7%) and the difference was ameliorated with antioxidant supplementation (10.7±1.9% vs. 10.8±1.8%), but the interaction was not significant (p=0.10). There were no significant changes in malondialdehyde-thiobarbituric acid reactive substances following antioxidant supplementation. There was a significant increase in brachial blood flow and forearm vascular conductance with exercise but no differences with antioxidant supplementation. There were no group differences in exercise responses and no differences with antioxidant supplementation, suggesting a lack of influence of oxidative stress during exercise in this cohort.

Key words

endothelial function - exercise blood flow - oxidative stress - antioxidant - racial differences - vascular

• References

• 1 Bassett Jr. DR, Duey WJ, Walker AJ, Howley ET, Bond V. Racial differences in maximal vasodilatory capacity of forearm resistance vessels in normotensive young adults. Am J Hypertens 1992; 5: 781-786
• 2 Cai H, Harrison DG. Endothelial dysfunction in cardiovascular diseases: the role of oxidant stress. Circ Res 2000; 87: 840-844
• 3 Campia U, Choucair WK, Bryant MB, Waclawiw MA, Cardillo C, Panza JA. Reduced endothelium-dependent and -independent dilation of conductance arteries in African Americans. J Am Coll Cardiol 2002; 40: 754-760
• 4 Cardillo C, Kilcoyne CM, Cannon 3^rd RO, Panza JA. Attenuation of cyclic nucleotide-mediated smooth muscle relaxation in blacks as a cause of racial differences in vasodilator function. Circulation 1999; 99: 90-95
• 5 Cardillo C, Kilcoyne CM, Cannon 3^rd RO, Panza JA. Racial differences in nitric oxide-mediated vasodilator response to mental stress in the forearm circulation. Hypertension 1998; 31: 1235-1239
• 6 Casey DP, Mohamed EA, Joyner MJ. Role of nitric oxide and adenosine in the onset of vasodilation during dynamic forearm exercise. Eur J Appl Physiol 2013; 113: 295-303
• 7 Corretti MC, Anderson TJ, Benjamin EJ, Celermajer D, Charbonneau F, Creager MA, Deanfield J, Drexler H, Gerhard-Herman M, Herrington D, Vallance P, Vita J, Vogel R. Guidelines for the ultrasound assessment of endothelial-dependent flow-mediated vasodilation of the brachial artery: a report of the International Brachial Artery Reactivity Task Force. J Am Coll Cardiol 2002; 39: 257-265
• 8 Diaz KM, Feairheller DL, Sturgeon KM, Williamson ST, Brown MD. Oxidative Stress Response to Short Duration Bout of Submaximal Aerobic Exercise in Healthy Young Adults. Int J Exerc Sci 2011; 4: 247-256
• 9 Dinenno FA, Masuki S, Joyner MJ. Impaired modulation of sympathetic alpha-adrenergic vasoconstriction in contracting forearm muscle of ageing men. J Physiol 2005; 567: 311-321
• 10 Djordjevic DZ, Cubrilo DG, Barudzic NS, Vuletic MS, Zivkovic VI, Nesic M, Radovanovic D, Djuric DM, Jakovljevic V. Comparison of blood pro/antioxidant levels before and after acute exercise in athletes and non-athletes. Gen Physiol Biophys 2012; 31: 211-219
• 11 Donato AJ, Uberoi A, Bailey DM, Wray DW, Richardson RS. Exercise-induced brachial artery vasodilation: effects of antioxidants and exercise training in elderly men. Am J Physiol 2010; 298: H671-H678
• 12 Eskurza I, Monahan KD, Robinson JA, Seals DR. Effect of acute and chronic ascorbic acid on flow-mediated dilatation with sedentary and physically active human ageing. J Physiol 2004; 556: 315-324
• 13 Faraci FM, Didion SP. Vascular protection: superoxide dismutase isoforms in the vessel wall. Arterioscler Thromb Vasc Biol 2004; 24: 1367-1373
• 14 Feairheller DL, Diaz KM, Sturgeon KM, Williamson ST, Brown MD. Racial Differences in the Time-Course Oxidative Stress Responses to Acute Exercise. J Exerc Physiol Online 2011; 14: 49-59
• 15 Feairheller DL, Park JY, Rizzo V, Kim B, Brown MD. Racial differences in the responses to shear stress in human umbilical vein endothelial cells. Vasc Health Risk Manag 2011; 7: 425-431
• 16 Feairheller DL, Park JY, Sturgeon KM, Williamson ST, Diaz KM, Veerabhadrappa P, Brown MD. Racial differences in oxidative stress and inflammation: in vitro and in vivo. Clin Transl Sci 2011; 4: 32-37
• 17 Go AS, Mozaffarian D, Roger VL, Benjamin EJ, Berry JD, Blaha MJ, Dai S, Ford ES, Fox CS, Franco S, Fullerton HJ, Gillespie C, Hailpern SM, Heit JA, Howard VJ, Huffman MD, Judd SE, Kissela BM, Kittner SJ, Lackland DT, Lichtman JH, Lisabeth LD, Mackey RH, Magid DJ, Marcus GM, Marelli A, Matchar DB, McGuire DK, Mohler 3^rd ER, Moy CS, Mussolino ME, Neumar RW, Nichol G, Pandey DK, Paynter NP, Reeves MJ, Sorlie PD, Stein J, Towfighi A, Turan TN, Virani SS, Wong ND, Woo D, Turner MB. Heart disease and stroke statistics – 2014 update: a report from the American Heart Association. Circulation 2014; 129: e28-e292
• 18 Gokce N, Keaney Jr. JF, Frei B, Holbrook M, Olesiak M, Zachariah BJ, Leeuwenburgh C, Heinecke JW, Vita JA. Long-term ascorbic acid administration reverses endothelial vasomotor dysfunction in patients with coronary artery disease. Circulation 1999; 99: 3234-3240
• 19 Gower BA, Ard JD, Hunter GR, Fernandez J, Ovalle F. Elements of the metabolic syndrome: association with insulin sensitivity and effects of ethnicity. Metab Syndr Relat Disord 2007; 5: 77-86
• 20 Granger HJ, Goodman AH, Granger DN. Role of resistance and exchange vessels in local microvascular control of skeletal muscle oxygenation in the dog. Circ Res 1976; 38: 379-385
• 21 Harris RA, Nishiyama SK, Wray DW, Tedjasaputra V, Bailey DM, Richardson RS. The effect of oral antioxidants on brachial artery flow-mediated dilation following 5 and 10 min of ischemia. Eur J Appl Physiol 2009; 107: 445-453
• 22 Harriss DJ, Atkinson G. Ethical Standards in Sport and Exercise Science Research: 2016 Update. Int J Sports Med 2015; 36: 1121-1124
• 23 Heffernan KS, Jae SY, Fernhall B. Racial differences in arterial stiffness after exercise in young men. Am J Hypertens 2007; 20: 840-845
• 24 Heffernan KS, Jae SY, Wilund KR, Woods JA, Fernhall B. Racial differences in central blood pressure and vascular function in young men. Am J Physiol 2008; 295: H2380-H2387
• 25 Heitzer T, Schlinzig T, Krohn K, Meinertz T, Munzel T. Endothelial dysfunction, oxidative stress, and risk of cardiovascular events in patients with coronary artery disease. Circulation 2001; 104: 2673-2678
• 26 Hozawa A, Folsom AR, Sharrett AR, Chambless LE. Absolute and attributable risks of cardiovascular disease incidence in relation to optimal and borderline risk factors: comparison of African American with white subjects – Atherosclerosis Risk in Communities Study. Arch Intern Med 2007; 167: 573-579
• 27 Irace C, Ceravolo R, Notarangelo L, Crescenzo A, Ventura G, Tamburrini O, Perticone F, Gnasso A. Comparison of endothelial function evaluated by strain gauge plethysmography and brachial artery ultrasound. Atherosclerosis 2001; 158: 53-59
• 28 Jablonski KL, Seals DR, Eskurza I, Monahan KD, Donato AJ. High-dose ascorbic acid infusion abolishes chronic vasoconstriction and restores resting leg blood flow in healthy older men. J Appl Physiol 2007; 103: 1715-1721
• 29 Kalinowski L, Dobrucki IT, Malinski T. Race-specific differences in endothelial function: predisposition of African Americans to vascular diseases. Circulation 2004; 109: 2511-2517
• 30 Lawrenson L, Poole JG, Kim J, Brown C, Patel P, Richardson RS. Vascular and metabolic response to isolated small muscle mass exercise: effect of age. Am J Physiol 2003; 285: H1023-H1031
• 31 Liu Y, Zhao H, Li H, Kalyanaraman B, Nicolosi AC, Gutterman DD. Mitochondrial sources of H2O2 generation play a key role in flow-mediated dilation in human coronary resistance arteries. Circ Res 2003; 93: 573-580
• 32 Loehr LR, Espeland MA, Sutton-Tyrrell K, Burke GL, Crouse 3^rd JR, Herrington DM. Racial differences in endothelial function in postmenopausal women. Am Heart J 2004; 148: 606-611
• 33 Minor DS, Wofford MR, Jones DW. Racial and ethnic differences in hypertension. Curr Atheroscler Rep 2008; 10: 121-127
• 34 Morris AA, Patel RS, Binongo JN, Poole J, Al Mheid I, Ahmed Y, Stoyanova N, Vaccarino V, Din-Dzietham R, Gibbons GH, Quyyumi A. Racial differences in arterial stiffness and microcirculatory function between Black and White Americans. J Am Heart Assoc 2013; 2: e002154
• 35 Perticone F, Ceravolo R, Pujia A, Ventura G, Iacopino S, Scozzafava A, Ferraro A, Chello M, Mastroroberto P, Verdecchia P, Schillaci G. Prognostic significance of endothelial dysfunction in hypertensive patients. Circulation 2001; 104: 191-196
• 36 Pescatello LS, Bairos L, Vanheest JL, Maresh CM, Rodriguez NR, Moyna NM, DiPasquale C, Collins V, Meckes CL, Krueger L, Thompson PD. Postexercise hypotension differs between white and black women. Am Heart J 2003; 145: 364-370
• 37 Richardson RS, Donato AJ, Uberoi A, Wray DW, Lawrenson L, Nishiyama S, Bailey DM. Exercise-induced brachial artery vasodilation: role of free radicals. Am J Physiol 2007; 292: H1516-H1522
• 38 Schisterman EF, Faraggi D, Browne R, Freudenheim J, Dorn J, Muti P, Armstrong D, Reiser B, Trevisan M. TBARS and cardiovascular disease in a population-based sample. J Cardiovasc Risk 2001; 8: 219-225
• 39 Schrage WG, Wilkins BW, Dean VL, Scott JP, Henry NK, Wylam ME, Joyner MJ. Exercise hyperemia and vasoconstrictor responses in humans with cystic fibrosis. J Appl Physiol 2005; 99: 1866-1871
• 40 Stein CM, Lang CC, Nelson R, Brown M, Wood AJ. Vasodilation in black Americans: attenuated nitric oxide-mediated responses. Clin Pharmacol Ther 1997; 62: 436-443
• 41 Stocker R, Keaney Jr JF. Role of oxidative modifications in atherosclerosis. Physiol Rev 2004; 84: 1381-1478
• 42 Suwaidi JA, Hamasaki S, Higano ST, Nishimura RA, Holmes Jr. DR, Lerman A. Long-term follow-up of patients with mild coronary artery disease and endothelial dysfunction. Circulation 2000; 101: 948-954
• 43 Taddei S, Virdis A, Ghiadoni L, Salvetti G, Bernini G, Magagna A, Salvetti A. Age-related reduction of NO availability and oxidative stress in humans. Hypertension 2001; 38: 274-279
• 44 Thijssen DH, Black MA, Pyke KE, Padilla J, Atkinson G, Harris RA, Parker B, Widlansky ME, Tschakovsky ME, Green DJ. Assessment of flow-mediated dilation in humans: a methodological and physiological guideline. Am J Physiol 2011; 300: H2-12
• 45 Tschakovsky ME, Sujirattanawimol K, Ruble SB, Valic Z, Joyner MJ. Is sympathetic neural vasoconstriction blunted in the vascular bed of exercising human muscle?. J Physiol 2002; 541: 623-635
• 46 Wilson JR, Kapoor SC. Contribution of prostaglandins to exercise-induced vasodilation in humans. Am J Physiol 1993; 265: H171-H175
• 47 Wray DW, Nishiyama SK, Harris RA, Zhao J, McDaniel J, Fjeldstad AS, Witman MA, Ives SJ, Barrett-O’Keefe Z, Richardson RS. Acute reversal of endothelial dysfunction in the elderly after antioxidant consumption. Hypertension 2012; 59: 818-824
• 48 Wray DW, Uberoi A, Lawrenson L, Bailey DM, Richardson RS. Oral antioxidants and cardiovascular health in the exercise-trained and untrained elderly: a radically different outcome. Clin Sci (Lond) 2009; 116: 433-441
• 49 Wray DW, Witman MA, Ives SJ, McDaniel J, Fjeldstad AS, Trinity JD, Conklin JD, Supiano MA, Richardson RS. Progressive handgrip exercise: evidence of nitric oxide-dependent vasodilation and blood flow regulation in humans. Am J Physiol 2011; 300: H1101-H1107
• 50 Yan H, Ranadive SM, Heffernan KS, Lane AD, Kappus RM, Cook MD, Wu PT, Sun P, Harvey IS, Woods JA, Wilund KR, Fernhall B. Hemodynamic and arterial stiffness differences between African-Americans and Caucasians after maximal exercise. Am J Physiol 2014; 306: H60-H68
• 51 Zion AS, Bond V, Adams RG, Williams D, Fullilove RE, Sloan RP, Bartels MN, Downey JA, De Meersman RE. Low arterial compliance in young African-American males. Am J Physiol 2003; 285: H457-H462