Antimikrobielle Oberflächenbeschichtung tötet multiresistente Krankheitserreger

Durch den dramatischen Anstieg an multiresistenten Krankheitserregern wird der Bedarf an neuen Agenzien zur Vermeidung von mikrobiellen Infektionen immer größer. Eine Möglichkeit stellen mit Silber beschichte Oberflächen oder Silbernanopartikel dar. Viele Mikroorganismen sind aber bereits silberresistent und die Zytotoxizität von Nanopartikeln erschwert deren Einsatz in der Medizin. Eine Alternative stellt die aus Silber und Ruthenium bestehende, mit einem Vitaminderivat konditionierte Oberflächenbeschichtung AGXX^® dar, die durch die Produktion von reaktiven Sauerstoffspezies Mikroorganismen effektiv abtötet. Resistenzen dagegen sind nicht beobachtet worden. Wir konnten zeigen, dass diese Beschichtung multiresistente Krankheitserreger wie MRSA, methicillinresistente Staphylococcus aureus, abtötet und dessen Biofilmbildung stark reduziert beziehungsweise verhindert. Auf Basis von quantitativen Reverse-Transkription-PCR-Daten (RT-qPCR-Daten) postulieren wir einen Reaktionsmechanismus für die Inhibition des Biofilmwachstums.

Due to the dramatic increase of the number of multi-resistant pathogens the demand of alternative agents to antibiotics to avoid microbial infections is steadily increasing. The use of silver-coated surfaces or silver nanoparticles is an option. However, many bacteria have developed silver resistance, and the cytotoxicity of nanoparticles causes problems in medical therapy. An alternative is AGXX^®, a surface coating consisting of silver and ruthenium, conditioned with a vitamin-derivative, which produces reactive oxygen species that effectively kill diverse microorganisms. Resistance against it has not been observed so far. We could show that this coating not only kills multiresistant pathogens such as MRSA, methicillin resistant Staphylococcus aureus, but also strongly reduces or even inhibits its biofilm formation. Based on quantitative Reverse Transcription PCR (RT qPCR) data we postulate a reaction mechanism for the inhibition of biofilm growth.

Key words

antimicrobial coating - silver - ruthenium - MRSA - biofilm

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