Planta Med 2021; 87(15): 1309-1310
DOI: 10.1055/s-0041-1736973
Abstracts
8. Poster Contributions
8.9 Recent Advances in Medicinal Plant and Natural Product Research

Oleanane triterpenoid saponins from Millettia pulchra radix possessing protective effect on paraquat-induced neurotoxicity

Thanh-Hoa Vo
1   School of Medicine, Vietnam National University Ho Chi Minh City, Ho Chi Minh City 700000, Vietnam
2   National Research Institute of Chinese Medicine, Ministry of Health and welfare, Taipei 11221, Taiwan
,
Chia-Ching Liaw
2   National Research Institute of Chinese Medicine, Ministry of Health and welfare, Taipei 11221, Taiwan
,
Yu-Chi Lin
2   National Research Institute of Chinese Medicine, Ministry of Health and welfare, Taipei 11221, Taiwan
,
DucHanh Nguyen
3   Department of Pharmaceutics, Faculty of Pharmacy, University of Medicine and Pharmacy at Ho Chi Minh City, Ho Chi Minh City 700000, Vietnam
,
Nai-Kuei Huang
2   National Research Institute of Chinese Medicine, Ministry of Health and welfare, Taipei 11221, Taiwan
,
Ching-Kuo Lee
4   School of Pharmacy, College of Pharmacy, Taipei Medical University, Taipei 11031, Taiwan.
,
Yao-Haur Kuo
2   National Research Institute of Chinese Medicine, Ministry of Health and welfare, Taipei 11221, Taiwan
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    After biological activity screening, the n-butanol layer partitioned from the ethanol extract of Millettia pulchra Kurz radix proved a protective effect on paraquat (PQ)-induced neurotoxicity. Further purification of the potential n-butanol layer by Diaion HP20 column, ODS open column chromatography, and reversed-phase preparative HPLC chromatography, three undescribed oleanane-type saponin derivatives (1-3), along with five known flavonoids (4-8), were isolated. The structures of isolated compounds were elucidated by ESI-MS, 1D, and 2D MNR data. The sugar units of saponins were determined by combined use of aldoimidazole derivatization and mass spectrometric analysis. The biological evaluation revealed that 1 possessed significant preventing PQ-induced neurotoxicity (IC50 3.0±0.05 µM).

    Keywords: Millettia pulchra Kurz, oleananes saponin, paraquat-induced neurotoxicity.


     

    The Authors declare no competing financial interests.

    Publication History

    Article published online:
    13 December 2021

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