Oleanane triterpenoid saponins from Millettia pulchra radix possessing protective effect on paraquat-induced neurotoxicity

After biological activity screening, the n-butanol layer partitioned from the ethanol extract of Millettia pulchra Kurz radix proved a protective effect on paraquat (PQ)-induced neurotoxicity. Further purification of the potential n-butanol layer by Diaion HP20 column, ODS open column chromatography, and reversed-phase preparative HPLC chromatography, three undescribed oleanane-type saponin derivatives (1-3), along with five known flavonoids (4-8), were isolated. The structures of isolated compounds were elucidated by ESI-MS, 1D, and 2D MNR data. The sugar units of saponins were determined by combined use of aldoimidazole derivatization and mass spectrometric analysis. The biological evaluation revealed that 1 possessed significant preventing PQ-induced neurotoxicity (IC₅₀ 3.0±0.05 µM).

Keywords: Millettia pulchra Kurz, oleananes saponin, paraquat-induced neurotoxicity.

Publikationsverlauf

Artikel online veröffentlicht:
13. Dezember 2021