Bioassay guided fractionation of Senna singueana and its potential for development of poultry phytogenic feed additives

Plant based products are potential alternatives to in-feed antibiotics. The aim of this study was to evaluate antibacterial, anti-lipoxygenase and antioxidant activity, and cytotoxicity of Senna singueana fractions and isolated compounds.

Solvent-solvent partitioning of the methanol leaf extract of S. singueana was followed by column chromatography with the ethyl acetate fraction. Antibacterial efficacy was determined against poultry pathogens including Escherichia coli, Staphylococcus aureus and Salmonella enteritidis using a two-fold serial microdilution assay. Anti-lipoxygenase activity was evaluated using the ferrous oxidation-xylenol orange (FOX) method. Antioxidant activity was assessed using radical scavenging assays. Fractions had significant to weak antibacterial activity with MIC values ranging from 50 – 938 µg/ml.

The ethyl acetate fraction and the seven subsequent column fractions had potent anti-lipoxygenase activity with IC₅₀ values of≤2.5 µg/ml, lower than that of quercetin (positive control). The ethyl acetate fraction and some sub-fractions had powerful antioxidant activity with IC₅₀ values of≤5 µg/ml in the ABTS assay. Bioassay-guided isolation led to isolation of a bioactive compound, luteolin.

Based on the good antibacterial activity, potent antioxidant and anti-inflammatory activity as well as low cytotoxicity of its fractions, S. singueana is a promising candidate for the development of poultry phytogenic feed additives.

 

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The authors declare no conflict of interest.

Publication History

Article published online:
13 December 2021