Evaluation of endophytes on Albizia julibrissin DURAZZ. from wild collection

The aim of our research is to discover new, plant-based alternatives for the treatment of chronic inflammatory diseases, from the treasures of traditional herbal medicines. [1] The bark extracts of Albizia julibrissin Durazz., showed anti-inflammatory activity in LPS- and IFNγ- induced RAW 264.7 macrophages and BV2 microglia cells in a screening phase, however the active compounds have not yet been identified. It has been reported, that Albiziae cortex is usually covered in patches of lichen and in the last few years, several plant endophytes have drawn considerable attention as a source of biologically active compounds. [2] To identify possible endophytes of A. julibrissin bark and their potential influence on the anti-inflammatory activity of

Albiziae cortex, samples from wild collection were dissected, surface sterilized and incubated at 28°C on different growth media. [3] Hyphae from the primary inoculation were picked and transferred to new agar plates to obtain a pure culture, which was used for direct PCR. Blast analysis was done to assign endophyte strains. Phytochemical analysis of active constituents is currently in progress.

• References

• 1 Waltenberger, B. et al. 2016. Monatshefte für Chemie - Chemical Monthly, 147(3), pp. 479–491. doi:10.1007/s00706-015-1653-y.
• 2 Rai, N. et al. 2021. Mycology, 12(3), pp. 139–159. doi:10.1080/21501203.2020.1870579
• 3 Sharma, N. et al. 2018. Journal of Applied Microbiology, 125(1), pp. 111–120. doi:10.1111/jam.13764

Publication History

Article published online:
13 December 2021