Thrombosis and Haemostasis, Table of Contents Thromb Haemost 1965; 13(01): 149-154DOI: 10.1055/s-0038-1656221 Originalarbeiten — Original Articles — Travaux Originaux Schattauer GmbH Schattauer Method for Determining Antiheparin Activity of Platelets and Erythrocytes A Poplawski 1 Department of Physiological Chemistry, Medical School, Bialystoh, Poland , S Niewiarowski 1 Department of Physiological Chemistry, Medical School, Bialystoh, Poland › Author Affiliations Recommend Article Abstract Buy Article Summary A method for determining antiheparin activity of platelets and erythrocytes is described. The neutralization of various concentrations of heparin by various amounts of protamine sulphate or tested material is measured simultaneously. Results are expressed in mean equivalent of protamine sulphate (PS) standard solution. The normal values of antiheparin activity of platelets and erythrocytes in human and in various animals are given. Full Text References References 1 Van Creveld S, Paulssen M. M. Isolation and properties of the third clotting factor in blood platelets. Lancet I: 23 1952; 2 Deutsch E. Differentiation of certain platelet factors in relation to blood coagulation. Rev. Hemat. 9: 483 1954; 3 Deutsch E. Eine einfache Methode zur Bestimmung von Thrombozytenfaktor 4. Thrombos. Diathes. haemorrh. (Stuttg.) 6: 93 1959; 4 Deutsch E, Wawersich E, Franke G. Über das Vorkommen eines Antiheparinfaktors in Thrombozyten und Geweben. I. Mitteilung Antiheparinaktivität der Thrombozyten. Thrombos. Diathes. haemorrh. (Stuttg.) 1: 3 1957; 5 Deutsch E, Wawersich E, Franke G. Über das Vorkommen eines Antiheparinfaktors in Thrombozyten und Geweben. II. Mitteilung Antiheparinaktivität von Hirnextrakten. Thrombos. Diathes. haemorrh. (Stuttg.) 1: 12 1957; 6 Deutsch E, Kain W. Studies on platelet factor 4. Henry Ford Hospital International Symposium. Blood Platelets, 1960, p. 337 7 Gastpar H, Goossens N. Eine gerinnungsphysiologische Bestimmungsmethode der Plättchenfaktor 4 Konzentration in Serum. 7th European Congress of Hematology, London, 1959, No 209 8 Hoffmeister K. L, Perlick E. Nachweis eines Antiheparinfaktors in der gesunden und der arteriosklerotischen Gefäβwand. Thrombos. Diathes. haemorrh. (Stuttg.) 6: 365 1961; 9 Holger-Maasen T. Reduction of heparin activity by plasma globulin in patients with increased heparin resistance. Acta haemat. (Basel) 27: 157 1962; 10 Lewis J. H, Ferguson J. H. Partial purification of human prothrombin and proconvertin; their characteristics and interaction. J. clin. Invest. 32: 915 1953; 11 Mayer G, Mayer S, Waitz R. Etude de l’activité antiheparinique des plaquettes par la méthode simple de Deutsch. Thrombos. Diathes. haemorrh. (Stuttg.) 9: 315 1963; 12 Niewiarowski S, Zywicka H, Latallo Z. Coagulation disorders in thrombocythaemia, a study of seven cases. Thrombos. Diathes. haemorrh. (Stuttg.) 7: 114 1962; 13 Poplawski A, Niewiarowski S. Dissociation of platelet antiheparin factor (platelet factor 4) and lipoprotein lipase inhibitor. Biochim. biophys. Acta 90: 403 1964; 14 Rapaport S. I, Ames S. B. Antiheparin activity of erythrocyte hemolysate. Proc. Soc. exp. Biol. 95: 158 1957; 15 Sokal G. Observations sur la morphologie et les fonctions plaquettaires dans les thrombo-pénies et les thrombocytoses. Rev. belge Path. 27: 103 1959; 16 Vandendriessche R. Separation of the thromboplastic activity and the heparin-neutralizing action of thrombocytes. Acta med. scand. 165: 217 1959;
