Further Purification of the Antihaemophilic Factor (AHF)

Summary

A description is given of the further purification of AHF by displacement chromatography on such materials as DEAE-Floc and DEAE-Sephadex. The use of a high ion-exchange capacity, which permits the use of relatively high salt concentrations, together with submaximal loading of the columns produced a high degree of purity of the AHF-preparations. The criteria of purity applied, and some properties of the AHF are described.

• References

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